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Merck
CN

03609

乙二胺四乙酸

BioUltra, ≥99.0% (KT)

别名:

EDTA, Ethylenedinitrilotetraacetic acid, 亚乙基二次氮基四乙酸, 依地酸

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关于此项目

线性分子式:
(HO2CCH2)2NCH2CH2N(CH2CO2H)2
化学文摘社编号:
分子量:
292.24
NACRES:
NA.25
PubChem Substance ID:
eCl@ss:
39030907
UNSPSC Code:
12352116
EC Number:
200-449-4
MDL number:
Beilstein/REAXYS Number:
1716295
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product line

BioUltra

Quality Level

assay

≥99.0% (KT)

form

powder

reaction suitability

reagent type: chelator

impurities

insoluble matter, passes filter test

pH

2.5 (23 °C, 10 g/L)

mp

250 °C (dec.) (lit.)

solubility

0.1 M NaOH: 0.1 M at 20 °C, clear, colorless

anion traces

chloride (Cl-): ≤50 mg/kg, sulfate (SO42-): ≤100 mg/kg

cation traces

Al: ≤10 mg/kg, As: ≤0.1 mg/kg, Ba: ≤5 mg/kg, Bi: ≤5 mg/kg, Ca: ≤10 mg/kg, Cd: ≤5 mg/kg, Co: ≤5 mg/kg, Cr: ≤5 mg/kg, Cu: ≤5 mg/kg, Fe: ≤5 mg/kg, K: ≤50 mg/kg, Li: ≤5 mg/kg, Mg: ≤5 mg/kg, Mn: ≤5 mg/kg, Mo: ≤5 mg/kg, Na: ≤500 mg/kg, Ni: ≤5 mg/kg, Pb: ≤5 mg/kg, Sr: ≤5 mg/kg, Zn: ≤5 mg/kg

λ

0.1 M in 1 M NaOH

UV absorption

λ: 260 nm Amax: ≤1.00, λ: 280 nm Amax: ≤0.05

SMILES string

OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O

InChI

1S/C10H16N2O8/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20)

InChI key

KCXVZYZYPLLWCC-UHFFFAOYSA-N

Application

乙二胺四乙酸 (EDTA) 是一种具有螯合二价阳离子(如钙和铯)能力的六齿配体和螯合剂。EDTA 广泛用于需要或使用隔离二价阳离子的领域。EDTA 用于人工限制性 DNA 切割工具的研究。

Other Notes

阴离子和金属阳离子的测定采用单柱色谱法,以 EDTA 为淋洗液,采用电导法和紫外检测法 ;毛细管电泳 DNA 酶切分析中的缓冲液组分


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signalword

Warning

存储类别

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

target_organs

Respiratory Tract

pictograms

Health hazardExclamation mark

Hazard Classifications

Acute Tox. 4 Inhalation - Eye Irrit. 2 - STOT RE 2 Inhalation

wgk

WGK 3



历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Yuichiro Aiba et al.
Chemical Society reviews, 40(12), 5657-5668 (2011-05-14)
This tutorial review provides recent developments in artificial cutters for site-selective scission of DNA with the focus on chemistry-based DNA cutters. They are useful tools for molecular biology and biotechnology, since their site-selectivity of scission is much higher than that
S. Matsushita
Journal of Chromatography A, 312, 327-327 (1984)
A Paulus et al.
Electrophoresis, 14(1-2), 27-35 (1993-01-01)
DNA digest analysis with polymer-filled capillaries in capillary electrophoresis is described. The samples analyzed consisted of commercially available standards including a 100 base pair ladder with repeating units up to 2000 base pairs. Three DNA digests covered the most common



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