16669
Magenta-Gal
reagent for selection of recombinant bacterial clones
别名:
5-Bromo-6-chloro-3-indolyl-β-D-galactopyranoside, Mag-Gal
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关于此项目
经验公式（希尔记法）:
C14H15BrClNO6
化学文摘社编号:
分子量:
408.63
PubChem Substance ID:
UNSPSC Code:
12352200
Beilstein/REAXYS Number:
1552602
MDL number:
grade
Molecular Biology
Quality Level
sterility
non-sterile
assay
≥98.0% (HPLC)
form
powder
solubility
DMF: soluble, DMSO: soluble
suitability
suitable for β-galactosidase test
storage temp.
−20°C
SMILES string
OC[C@H]1O[C@@H](Oc2c[nH]c3cc(Cl)c(Br)cc23)[C@H](O)[C@@H](O)[C@@H]1O
InChI
1S/C14H15BrClNO6/c15-6-1-5-8(2-7(6)16)17-3-9(5)22-14-13(21)12(20)11(19)10(4-18)23-14/h1-3,10-14,17-21H,4H2/t10-,11-,12+,13-,14-/m1/s1
InChI key
CHRVKCMQIZYLNM-RKQHYHRCSA-N
General description
Magenta-Gal is a chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. Magenta-Gal is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype.
Application
Suitable for use in selection of recombinant bacterial colonies with the lac+ phenotype. Also suitable for use immunoblotting and immunocytochemical assays.
Biochem/physiol Actions
When Magenta-Gal is hydrolyzed by β-galactosidase, the resulting product will form a red precipitate. Lac+ colonies grown in the presence of Magenta-Gal turn an intense red color, allowing for easy differentiation between lac+ and lac- colonies.
存储类别
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
新产品
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Yeon-Jin Kim et al.
Developmental biology, 397(1), 129-139 (2014-12-03)
Members of the fibroblast growth factor (FGF) family play important roles during various developmental processes including eye development. FRS (FGF receptor substrate) proteins bind to FGFR and serve as adapters for coordinated assembly of multi-protein complexes involved in Ras/MAPK and