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关于此项目
化学文摘社编号:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
235-747-3
Specific activity:
~100000 U/mg
Biological source:
chicken egg white
biological source
chicken egg white
Quality Level
form
powder
quality
dialyzed, lyophilized
specific activity
~100000 U/mg
mol wt
single-chain 14.3 kDa, Mr ~14600
technique(s)
cell based assay: suitable
suitability
suitable for cell lysis
UniProt accession no.
application(s)
cell analysis
storage temp.
2-8°C
Gene Information
chicken ... LYZ(396218)
Application
来自鸡蛋清的溶菌酶已被用于:
- 作为小角度中子散射实验 和红外吸收光谱的标准品
- 在蛋白质结晶实验中,作为细胞裂解缓冲液的组分
酶可分解细菌的细胞壁;用于制备原生质体。
Biochem/physiol Actions
溶菌酶可水解肽聚糖中N-乙酰胞壁酸与N-乙酰-D-氨基葡萄糖残基之间以及壳糊精中N-乙酰-D-氨基葡萄糖残基之间的β(1→4)连接。 革兰氏阳性细胞对这种水解非常敏感,因为它们的细胞壁中的肽聚糖比例很高。革兰氏阴性菌就对这种水解没那么敏感,因为它们有外膜并且肽聚糖的比例较低。然而,这些细胞可能在细胞外膜中螯合金属离子的EDTA存在的情况下水解。
该酶在广泛的pH范围(6.0至9.0)内具有活性。pH值为6.2时,可在更广的离子强度范围(0.02至0.100 M)观察到最大活性,而pH值为9.2时为0.01至0.06 M。
该酶在广泛的pH范围(6.0至9.0)内具有活性。pH值为6.2时,可在更广的离子强度范围(0.02至0.100 M)观察到最大活性,而pH值为9.2时为0.01至0.06 M。
溶菌酶可水解肽聚糖中N-乙酰胞壁酸与N-乙酰-D-氨基葡萄糖残基之间以及壳糊精中N-乙酰-D-氨基葡萄糖残基之间的β(1→4)连接。革兰氏阳性细胞对这种水解非常敏感,因为它们的细胞壁中的肽聚糖比例很高。
Other Notes
1 U对应于在pH7.0和25°C下,将酶在450nm处的吸光度降低0.001(每分钟0.001)所需的酶量[藤黄微球菌(Micrococcus luteus),ATCC 4698,作为底物]
适用于细菌细胞壁、粘多糖、粘多肽或几丁质的水解;可降解乳酸乳球菌乳脂亚种H2细胞壁;综述
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Explore all of our products under Lysozyme 来源于鸡蛋白
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
动植物源性产品
低风险生物材料
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