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Merck
CN

93283

Tris-EDTA 缓冲液

BioUltra, Molecular Biology, pH 8.0

别名:

TE缓冲液, TE 缓冲液 溶液

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关于此项目

UNSPSC Code:
41105319
eCl@ss:
32129211
PubChem Substance ID:
NACRES:
NA.25
MDL number:
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grade

Molecular Biology

product line

BioUltra

form

liquid

impurities

DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected

pH

8.0±0.2 (25 °C)

cation traces

Al: ≤1 mg/kg, Ba: ≤1 mg/kg, Bi: ≤1 mg/kg, Ca: ≤5 mg/kg, Cd: ≤1 mg/kg, Co: ≤1 mg/kg, Cr: ≤1 mg/kg, Cu: ≤1 mg/kg, Fe: ≤1 mg/kg, K: ≤20 mg/kg, Li: ≤1 mg/kg, Mg: ≤1 mg/kg, Mn: ≤1 mg/kg, Mo: ≤1 mg/kg, Ni: ≤1 mg/kg, Pb: ≤1 mg/kg, Sr: ≤1 mg/kg, Zn: ≤1 mg/kg

λ

neat

UV absorption

λ: 260 nm Amax: 0.04, λ: 280 nm Amax: 0.01

application(s)

sample preparation

SMILES string

NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O

InChI

1S/C10H16N2O8.C4H11NO3/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2

InChI key

VLEIUWBSEKKKFX-UHFFFAOYSA-N

General description

Tris EDTA是生物学研究中的一种缓冲液。它是Tris碱和EDTA(一种金属螯合剂)的组合。Tris提供缓冲能力,可在生物样本中维持稳定的pH值,而EDTA则通过螯合二价金属离子来防止金属离子的干扰。Tris EDTA常用于分子生物学技术中,如DNA和RNA样本制备、电泳和酶促反应。它通过抑制核酸酶和金属依赖性酶的活性,帮助维持核酸的稳定性和完整性。

Application

TE缓冲液在一般提取、悬浮、洗涤和储存缓冲液以及DNA或RNA的制备中很有用。它通常用于DNA测序、限制性酶消化、连接和聚合酶链式反应(PCR)等应用。

Features and Benefits

  • BioUltra级别 - 适用于许多分子生物学应用
  • 无DNase、RNase、磷酸酶和蛋白酶
  • 高纯度,无微量金属。

Other Notes

如需了解生化试剂系列的更多信息,请填写此表


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存储类别

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves



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货号GTIN
93283-500ML04061833282533
93283-100ML04061838254573