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P2308

蛋白酶 K 来源于林伯氏白色念球菌

Name: 蛋白酶 K 来源于<I>林伯氏白色念球菌</I>
Brand: SIAL

lyophilized powder, Molecular Biology, BioUltra, ≥30 units/mg protein

别名:

肽链内切酶

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关于此项目

化学文摘社编号:

39450-01-6

UNSPSC Code:

12352204

eCl@ss:

32160410

EC Number:

254-457-8

NACRES:

NA.54

MDL number:

MFCD00132129

EC 号:

3.4.21.64 (BRENDA, IUBMB)

Specific activity:

≥30 units/mg protein

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属性

grade

Molecular Biology

product line

BioUltra

form

lyophilized powder

specific activity

≥30 units/mg protein

mol wt

28.93 kDa

composition

Protein, ≥90% biuret

impurities

≤1 ppm DNA

foreign activity

DNAse, Nickase and RNAse, none detected

shipped in

wet ice

storage temp.

−20°C

说明

Application

在分离DNA和RNA的过程中，对核酸酶的蛋白水解失活起作用。

在分离DNA和RNA的过程中，用于核酸酶的蛋白水解失活。
用于去除内毒素结合的阳离子蛋白，如溶菌酶和核糖核酸酶A。
据报道，其可用于分离肝、酵母和绿豆线粒体
用于测定酶在细胞膜上的定位
用于石蜡包埋组织切片的处理，暴露用于抗体标记的抗原结合位点。
用于可传播海绵状脑病(TSE)研究中朊病毒脑组织样本蛋白的消化。

Biochem/physiol Actions

蛋白酶K是一种稳定且具有高活性的丝氨酸蛋白酶。晶体和分子结构研究证据表明该酶属于枯草素家族，具有活性位点催化三联体(Asp³⁹-His⁶⁹-Ser²²⁴)。它在一系列广泛的环境中均稳定：pH，缓冲盐，去污剂（SDS）以及温度。在0.1-0.5% SDS存在的情况下，蛋白酶K可保持活性，在不损害分离DNA完整性的前提下，可以消化DNA制剂中的多种蛋白质和核酸酶。

Other Notes

在pH 7.5，37 °C 条件下，一单位的酶每分钟将会尿素变性的血红蛋白以产生与1.0 μ摩尔 (181 μg) 酪氨酸等同的颜色（Folin-Ciocalteu试剂提供的颜色）。

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pictograms

GHS08,GHS07

signalword

Danger

hcodes

H315,H319,H334,H335

pcodes

P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

存储类别

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

法规信息

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