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线性分子式:
K2HPO4 · 3H2O
化学文摘社编号:
分子量:
228.22
NACRES:
NA.25
PubChem Substance ID:
UNSPSC Code:
12161700
EC Number:
231-834-5
MDL number:
Quality Level
product line
ReagentPlus®
assay
≥99.0%
form
powder or crystals
pH
9.2-9.4 (25 °C, 50 g/L)
solubility
water: 100 mg/mL, clear, colorless
storage temp.
room temp
SMILES string
O.O.O.[K+].[K+].OP([O-])([O-])=O
InChI
1S/2K.H3O4P.3H2O/c;;1-5(2,3)4;;;/h;;(H3,1,2,3,4);3*1H2/q2*+1;;;;/p-2
InChI key
XQGPKZUNMMFTAL-UHFFFAOYSA-L
General description
磷酸钾溶液具有非常高的缓冲能力,广泛用于分子生物学、生物化学和色谱应用。磷酸二氢钾三水合物是配制磷酸钾缓冲液、磷酸盐缓冲盐水 (PBS) 和磷酸盐缓冲 EDTA (PBE) 的关键成分。它广泛用于各种生物实验的生物样品制备。磷酸钾缓冲液还用于平衡透析、高效液相色谱(HPLC)和亲和毛细管电泳(ACE)等方法,以研究体内药物-蛋白质相互作用的特征。磷酸钾缓冲溶液由磷酸二氢钾和磷酸氢二钾的混合物组成。
Application
磷酸氢二钾三水合物可用于制备重悬聚电解质胶囊所需的磷酸钾缓冲液。它还还可用作谷氨酰胺酶‑1 (GLS-1)酶测定和 GLS-2 酶测定方案的缓冲液。
Legal Information
ReagentPlus is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
13 - Non Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
实验方案
在测定超氧化物歧化酶活性时,使用了一种在550 nm处的连续分光光度法进行测定。一个酶单位可将酶偶联系统的细胞色素c还原率抑制50%。
Enzymatic Assay of Superoxide Dismutase
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全球贸易项目编号
| 货号 | GTIN |
|---|---|
| P5504-100G | 04061835547760 |
| P5504-5KG | 04061834391791 |
| P5504-10KG | 04061833623282 |
| P5504-1KG | 04061835547777 |
| P5504-500G | 04061835547784 |