S9430
SYBR®Green I核酸凝胶染液
10,000 × in DMSO
别名:
DNA stain, SYBR® green gel dye, safer gel stain
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关于此项目
form
solution
Quality Level
usage
mL sufficient for 100 mini-gels
greener alternative product characteristics
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
concentration
10,000 × in DMSO
technique(s)
PCR: suitable
greener alternative category
storage temp.
−20°C
General description
SYBR® Green I是一种特制的不对称花菁染料，用于检测核酸。这种成分由N-烷基化苯并噻唑或苯并噁唑环系统组成，通过单次甲基桥连接吡啶鎓或喹啉鎓环系统。SYBR Green I与dsDNA的小沟结合，激发波长480 nm。其荧光发射峰波长为520nm。
Application
SYBR® Green I核酸凝胶染色剂已用于：
- dsDNA定量
- 聚合酶链式反应（PCR），用于DNA染色
- Comet测定技术
- 评估精子膜的完整性
- 扩增DNA 目视检查（环介导等温扩增（LAMP）法）
- 用作流式细胞术中的荧光染料
- 实时定量逆转录聚合酶链式反应（qRT-PCR），用于逆转录cDNA染色
Features and Benefits
- 用于在电泳后对琼脂糖或聚丙烯酰胺凝胶中的双链DNA染色的超灵敏染色剂
- 它还可以检测变性琼脂糖/甲醛和聚丙烯酰胺/尿素凝胶中的ssDNA和RNA，无需任何预洗涤步骤
- 在 Ames 试验中，它的致突变性比溴化乙锭小
- 它对寡核苷酸的检测灵敏度是溴化乙锭的 50-100 倍
- 适用于 DNA 数量有限的许多应用
- SYBR® Green I 与 DNA 的结合不会抑制许多常见限制性内切酶的活性，包括 Hind III 和 EcoR I
- 这种染色剂无需通过凝胶酶解和连接技术去除
- SYBR Green I 是溴化乙锭染色的绿色替代产品
Preparation Note
将产品储存在–20 °C。稀释后的染色溶液避光可在2–8 °C保存数周或室温保存3-4天。
Legal Information
SYBR is a registered trademark of Thermo Fisher Scientific or its subsidiaries
存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
201.2 °F - closed cup
flash_point_c
94 °C - closed cup
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
实验方案
面向全基因组扩增(WGA)的SeqPlexDNA扩增试剂盒(SEQXE)专用于对极小量或降解/高度片段化的DNA进行下一代测序(NGS)。
SeqPlex RNA扩增试剂盒可在进入常用深度测序平台工作流程之前为总RNA或分离后的mRNA提供一种扩增方法，
WTA2（一种全转录组扩增（WTA）方法）能够在区区4小时内，代表性地扩增出纳克级的总RNA而没有3'偏差。
商品
SeqPlex™-I WTA kit amplifies RNA for NGS, enabling genomic studies from limited samples.
Loop mediated isothermal amplification of 5.8S rDNA for specific
detection of Tritrichomonas foetus
detection of Tritrichomonas foetus
Jorge Oyhenart
Veterinary Parasitology, 193 (2013)
I Ortiz et al.
Animal reproduction science, 187, 74-78 (2017-10-19)
The aim of this study was to compare sperm DNA fragmentation of frozen-thawed epididymal sperm of dogs using the SCSA (Sperm Chromatin Structure Assay) and SCDt (Sperm Chromatin Dispersion test). For this purpose, epididymis from neutered dogs were minced and
P A Morin et al.
BioTechniques, 19(2), 223-228 (1995-08-01)
A method for resolving and visualizing genotypes for simple sequence repeat loci on short (20 cm) polyacrylamide gels is described. This method makes use of a modified vertical electrophoresis cell to allow rapid electrophoresis without variation in migration rates due
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| S9430-.5ML | 04061837899140 |
| S9430-1ML | 04061837899164 |