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Merck
CN

32211

氯仿

puriss. p.a., reag. ISO, reag. Ph. Eur., 99.0-99.4% (GC)

别名:

三氯甲烷

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关于此项目

经验公式(希尔记法):
CHCl3
化学文摘社编号:
分子量:
119.38
UNSPSC Code:
12191502
NACRES:
NA.04
PubChem Substance ID:
EC Number:
200-663-8
Beilstein/REAXYS Number:
1731042
MDL number:
Assay:
99.0-99.4% (GC)
Technique(s):
RNA extraction: suitable
Bp:
60.5-61.5 °C (lit.)
Vapor pressure:
160 mmHg ( 20 °C)
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Quality Level

agency

USP/NF, reag. ISO, reag. Ph. Eur.

vapor density

4.1 (vs air)

vapor pressure

160 mmHg ( 20 °C)

grade

puriss. p.a.

assay

99.0-99.4% (GC)

form

liquid

contains

~1% ethanol as stabilizer

technique(s)

RNA extraction: suitable

impurities

≤0.00001% free chlorine (Cl), ≤0.00005% free acid (as HCl), ≤0.0005% non-volatile matter, ≤0.005% aldehydes and ketones (as CH3COCH3), ≤0.005% carbonyl compounds (as CO), ≤0.01% tetrachloroethene (GC), ≤0.01% tetrachloromethane (GC), ≤0.01% trichloroethene (GC), ≤0.01% water (Karl Fischer), ≤0.03% dichloromethane (GC), 0.6-1.0% ethanol (GC)

refractive index

n20/D 1.445 (lit.)

bp

60.5-61.5 °C (lit.)

mp

−63 °C (lit.)

density

1.476-1.483 g/mL at 20 °C, 1.492 g/mL at 25 °C (lit.)

anion traces

chloride (Cl-): ≤1 mg/kg

SMILES string

ClC(Cl)Cl

cation traces

Al: ≤0.5 mg/kg, B: ≤0.02 mg/kg, Ba: ≤0.1 mg/kg, Ca: ≤0.5 mg/kg, Cd: ≤0.05 mg/kg, Co: ≤0.02 mg/kg, Cr: ≤0.02 mg/kg, Cu: ≤0.02 mg/kg, Fe: ≤0.1 mg/kg, Mg: ≤0.1 mg/kg, Mn: ≤0.02 mg/kg, Ni: ≤0.02 mg/kg, Pb: ≤0.05 mg/kg, Sn: ≤0.1 mg/kg, Zn: ≤0.1 mg/kg

suitability

complies for appearance, complies for reaction against H2SO4, complies for suitability of determ. w. dithizone

InChI

1S/CHCl3/c2-1(3)4/h1H

InChI key

HEDRZPFGACZZDS-UHFFFAOYSA-N

General description

Chloroform, a halogenated hydrocarbon, is widely employed as a solvent. Its photochemical degradation has been investigated in the presence of TiO2 aqueous suspensions (wavelength range of 310-380nm).

Application

Chloroform may be used as a solvent to enhance the enantioselectivity of secondary alcohols formed by the borohydride reduction of carbonyl compounds in the presence of optically active ketoiminatocobalt complexes.
Chloroform may be employed as a solvent for the following studies:
  • Extraction of RNA from plant cells.
  • Preparation of dioleoylphosphatidylcholine (DOPC) solution.
  • Dissolution of 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC).
It may be employed in a rapid quantitative method for the release of periplasmic proteins from bacterial strains.

Other Notes

For information on chloroform miscibility, please visit the following link:
Chloroform Miscibility/Immiscibility Table
The article number 32211-4X2.5L will be discontinued. Please order the single bottle 32211-2.5L which is physically identical with the same exact specifications.
The article number 32211-4X2.5L-M will be discontinued. Please order the single bottle 32211-2.5L-M which is physically identical with the same exact specifications.
The article number 32211-6X1L will be discontinued. Please order the single bottle 32211-1L which is physically identical with the same exact specifications.
The article number 32211-6X1L-M will be discontinued. Please order the single bottle 32211-1L-M which is physically identical with the same exact specifications.


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pictograms

Skull and crossbonesHealth hazard

signalword

Danger

Hazard Classifications

Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Carc. 2 - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2 - STOT RE 1 Oral - STOT SE 3

target_organs

Central nervous system, Liver,Kidney

存储类别

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

wgk

WGK 3

flash_point_f

does not flash

flash_point_c

does not flash

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Chloroform is not solvent but activator for cobalt complex catalyst of enantioselective borohydride reduction.
Kokura, Ai, et al.
Chemistry Letters (Jpn), 36(1), 26-27 (2006)
Gurman Singh Pall et al.
Nucleic acids research, 35(8), e60-e60 (2007-04-05)
The northern blot, or RNA gel blot, is a widely used method for the discovery, validation and expression analysis of small regulatory RNA such as small interfering RNA (siRNA), microRNA (miRNA) and piwi-interacting RNA (piRNA). Although it is straightforward and
G F Ames et al.
Journal of bacteriology, 160(3), 1181-1183 (1984-12-01)
We introduce a method by which periplasmic proteins can be released rapidly, simply, and quantitatively by treating cells with chloroform. All the amino acid-binding proteins tested maintained their activity during chloroform treatment. This method makes practical the analysis of the



全球贸易项目编号

货号GTIN
32211-144X1L04061837850776
32211-195L04061838069733
32211-1L04061826702796
32211-6X1L04061837850820
32211-2.5L04061837850790
32211-4X2.5L04061837850806