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08445

7-氨基-4-甲基香豆素-3-乙酸

Name: 7-氨基-4-甲基香豆素-3-乙酸
Brand: SIGMA

BioReagent, suitable for fluorescence, ~90% (HPLC)

别名:

2H-1-苯并吡喃-3-乙酸, 7-氨基-4-甲基-3-香豆素醋酸, AMCA酸

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关于此项目

经验公式（希尔记法）:

C₁₂H₁₁NO₄

化学文摘社编号:

106562-32-7

分子量:

233.22

NACRES:

NA.32

PubChem Substance ID:

329748585

UNSPSC Code:

12352106

MDL number:

MFCD00081051

Beilstein/REAXYS Number:

7927205

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属性

product line

BioReagent

Quality Level

100

assay

~90% (HPLC)

form

powder

solubility

DMF: soluble, DMSO: soluble, aqueous base: soluble

fluorescence

λ_ex 350 nm; λ_em 433 nm in methanol

suitability

suitable for fluorescence

InChI

1S/C12H11NO4/c1-6-8-3-2-7(13)4-10(8)17-12(16)9(6)5-11(14)15/h2-4H,5,13H2,1H3,(H,14,15)

InChI key

QEQDLKUMPUDNPG-UHFFFAOYSA-N

说明

Application

AMCA是一种用于标记蛋白的试剂。其在345 nm处具有特征吸收峰以及在440-460 nm处具有荧光发射（蓝色区域）；然而，当与蛋白质缀合时，吸收峰移至355 nm，并在440-460 nm处发射荧光。其具有高的荧光产量和足够的斯托克斯位移（100 nm），并且相对光稳定。其相对容易被激活并偶联至肽的N端氨基，从而形成酸稳定的酰胺键。

Packaging

无底玻璃瓶。内含物在插入的融合锥体内。

Other Notes

有效的蓝色荧光团，可用于免疫荧光， λ_abs ~350 nm, λ_em ~440 nm

pictograms

GHS07

signalword

Warning

hcodes

H315,H319,H335

pcodes

P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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商品

感应染料

一氧化氮 (NO) 是神经元、内皮细胞和免疫系统中的信号转运分子。

Sensor Dyes

Nitric oxide (NO) as a signal transporter in neurons, endothelial cells and in the immune system.

Immunodominance of a low-affinity major histocompatibility complex-binding myelin basic protein epitope (residues 111-129) in HLA-DR4 (B1*0401) subjects is associated with a restricted T cell receptor repertoire.

P A Muraro et al.

The Journal of clinical investigation, 100(2), 339-349 (1997-07-15)

The pathogenesis of multiple sclerosis (MS) is currently ascribed in part to a T cell-mediated process targeting myelin components. The T cell response to one candidate autoantigen, myelin basic protein (MBP), in the context of HLA-DR15Dw2, has been previously studied

Aminomethyl coumarin acetic acid: a new fluorescent labelling agent for proteins.

H Khalfan et al.

The Histochemical journal, 18(9), 497-499 (1986-09-01)

A new fluorescent protein labelling agent, 7-amino-4-methyl coumarin-3-acetic acid (AMCA), emits in the blue region (440-460 nm) on activation with UV light (350 nm). The active reagent is the N-hydroxysuccinimide ester which reacts with lysine residues under mild conditions to

Three-color fluorescence in situ hybridization for the simultaneous detection of multiple nucleic acid sequences.

P M Nederlof et al.

Cytometry, 10(1), 20-27 (1989-01-01)

A method is described for visualizing three nucleic acid sequences simultaneously by in situ hybridization using a new blue immunofluorescent label, amino methyl coumarin acetic acid (AMCA), in combination with green and red fluorescing FITC and TRITC. Three chromosome-specific repetitive

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