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Merck
CN

10065

α-淀粉酶 来源于米曲霉

powder, ~30 U/mg

别名:

1,4-α-D-葡聚糖-葡萄糖水解酶

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关于此项目

UNSPSC Code:
12352204
eCl@ss:
32160410
EC Number:
232-588-1
NACRES:
NA.54
MDL number:
Specific activity:
~30 U/mg
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form

powder

Quality Level

specific activity

~30 U/mg

mol wt

Mr ~51000

storage temp.

2-8°C

General description

微生物淀粉酶是胞外酶,可用于多种工业应用,如面包和麦芽糖浆生产、酱油和豆面酱发酵等。从米曲霉中分离的α-淀粉酶的分子量为51kDa(沉降和扩散)和49kDa(凝胶过滤)。

Application

α-淀粉酶已被用作
  • 琼脂平板和羧甲基纤维素清除试验的对照酶,用于筛选淀粉水解的纤维素酶活性,
  • 以便探讨小麦淀粉在冷冻面团中
  • 抑制金黄色葡萄球菌生物膜的作用。

Biochem/physiol Actions

米曲霉α-淀粉酶(Ao α-淀粉酶)酶催化可溶性淀粉和相关底物中α-1,4糖苷键的水解。这些底物被分解并释放较短的低聚糖和α-极限糊精。

Other Notes

1个酶活力单位(U)相当于在pH6.0和25℃下每分钟释放1μmol麦芽糖所需的酶量(淀粉符合Zulkowsky,货号85642,作为底物)。
用于测定食物中的脂肪;应用于糖苷键的(选择性)水解/缩合。


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pictograms

Health hazardExclamation mark

signalword

Danger

hcodes

Hazard Classifications

Acute Tox. 4 Oral - Resp. Sens. 1

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

法规信息

常规特殊物品

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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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S Wirsel et al.
Molecular microbiology, 3(1), 3-14 (1989-01-01)
We have cloned three genes (amy1, amy2 and amy3) encoding alpha-amylase in the filamentous fungus Aspergillus oryzae. The established overall sequences have a very high degree of homology, showing divergences mainly in the 3'-untranslated regions. The positions and the sequences
P C Sehnke et al.
Proceedings of the National Academy of Sciences of the United States of America, 98(2), 765-770 (2001-01-10)
In higher plants the production of starch is orchestrated by chloroplast-localized biosynthetic enzymes, namely starch synthases, ADP-glucose pyrophosphorylase, and starch branching and debranching enzymes. Diurnal regulation of these enzymes, as well as starch-degrading enzymes, influences both the levels and composition
C E Daugherty et al.
Journal - Association of Official Analytical Chemists, 66(4), 927-932 (1983-07-01)
A chloroform-methanol extraction method (complete extraction of fat in 3 min) for determining fat in processed and prepared foods has been studied collaboratively. Fourteen collaborators reported single replicate fat results on 7 samples representative of various food types and 2



全球贸易项目编号

货号GTIN
10065-10G04061835511242
10065-50G04061835550982