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10831

Histopaque®-1083

sterile-filtered, density: 1.083

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关于此项目

NACRES:
NA.75
UNSPSC Code:
12352207
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sterility

sterile-filtered

Quality Level

400

form

liquid

shelf life

Expiry date on the label.

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

density

1.083

application(s)

hematology
histology

storage temp.

2-8°C

General description

Histopaque®-1083是一种含有聚蔗糖和泛影酸钠的溶液，密度调整至1.083 g/mL。它有助于从大鼠、小鼠和其他小型哺乳动物的少量血液中分离活的单核细胞。

Application

Histopaque®-1083用于从大鼠外周血中分离单核细胞和白细胞。

Biochem/physiol Actions

将抗凝血液铺在HISTOPAQUE1083上。离心时，红细胞被聚多糖聚集并迅速沉淀，而淋巴细胞和其他单个核细胞保留在血浆-HISTOPAQUE1083分界面。红细胞会沉淀到离心管的底部。

Features and Benefits

Histopaque®-1083 有许多优势，比如：

  • 可使活细胞极佳恢复
  • 允许选择分离不同种类的血细胞
  • 防止细胞变形
  • 保持细胞活力
  • 最小化外来细胞干扰
  • 各批次性能可重复
  • 经过过滤杀菌和内毒素检测
  • 在 2-8°C 避光保护条件下可稳定保存至少2年

Legal Information

Histopaque is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Corrosion
GHS05

signalword

Warning

Hazard Classifications

Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

存储类别

10 - Combustible liquids

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
RNBL4897
RNBL5440
RNBK9776
RNBK6510
RNBK4686

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实验方案

用于梯度离心的Ficoll® 400

Ficoll 400®是由蔗糖和环氧氯丙烷共聚而成的一种高度分支化的聚合物。Ficoll 400®是完全非离子性的。由于其含有丰富的羟基，Ficoll 400®具有很强的亲水性和水溶性。

Histopaque® 密度梯度介质故障排除指南

本疑难排查指南针对使用 Histopaque 时观察到的最常见错误来源提出解决方案，但这并不代表本指南是全面无遗的。

Ficoll® 400 for Gradient Centrifugation

Ficoll 400® is a non-ionic polymer with high hydrophilicity and water solubility, suitable for various biological applications.

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商品

默克密度梯度介质_细胞分离-默克生命科学

我们提供完整的密度梯度介质产品线，用于分离或提取白细胞、病毒、DNA、RNA、细胞器以及许多其他应用。我们的产品线包括用于在研究实验室中分离白细胞的Histopaque®碘化梯度介质，用于哺乳动物细胞分离的多糖介质，用于哺乳动物细胞和细胞器分离的胶体二氧化硅介质，以及用于DNA、病毒和蛋白质分离的无机盐介质。

Centrifugation Basics

Centrifugation enables the separation of particles by sedimentation. Learn how to separate particles using a centrifuge and how to use Stokes' law to calculate the velocity of sedimentation.

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