conjugate
Atto 647N conjugate
Quality Level
antibody form
affinity isolated antibody
antibody product type
secondary antibodies
clone
polyclonal
contains
50% glycerol as stabilizer
species reactivity
mouse
concentration
≥0.8 mg/mL IgG
technique(s)
immunofluorescence: 5 μg/mL
fluorescence
λex 647 nm; λem 665 nm in PBS
suitability
in accordance for fluorescence
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
General description
免疫球蛋白G(IgG)是一种糖蛋白抗体,其可调节免疫反应,如吞噬作用,也可参与自身免疫疾病的发展。小鼠IgG具有四种不同的同种型,即IgG1、IgG2a、IgG2b和IgG3。IgG1可调节小鼠的补体结合。Atto 647N山羊抗小鼠IgG与小鼠IgG结合。
Immunogen
小鼠 IgG
Application
Atto荧光标记专门用于单分子检测等高灵敏度应用。 Atto标记的结构具有刚性,构型不会产生任何顺反异构变化。 因此这种标记偶联后的强度表现卓越,光谱转变却又极低。Atto 647N 山羊抗兔 IgG 已作为二抗(浓度为 5μg/ml),用于2% 甲醛固定细胞的免疫荧光。
成功使用该抗体的应用以及相关的同行评审论文如下所示。
蛋白质免疫印迹分析(1 篇论文)
蛋白质免疫印迹分析(1 篇论文)
Physical form
Atto 647 山羊抗小鼠 IgG(全分子)以 1 mg/ml 溶液的形式提供,溶剂为 0.1M 磷酸钠,0.1M NaCl,pH 7.5,含有 5 mM 叠氮化钠作为防腐剂。
Analysis Note
未缀合染料的总荧光 ≤5%
Legal Information
本产品仅供研究使用。如果要商业化,请联系知识产权持有者(ATTO-TEC GmbH,德国)获取许可。
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
法规信息
低风险生物材料
常规特殊物品
此项目有
商品
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全球贸易项目编号
| 货号 | GTIN |
|---|---|
| 50185-1ML-F | 04061838061997 |