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69898

溴乙烷

Name: 溴乙烷
Brand: SIGMA

suitable for fluorescence, BioReagent, ≥95% (HPCE)

别名:

单溴代二苯甲烷

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关于此项目

经验公式（希尔记法）:

C₁₀H₁₁BrN₂O₂

化学文摘社编号:

71418-44-5

分子量:

271.11

UNSPSC Code:

12171500

NACRES:

NA.32

PubChem Substance ID:

57652223

MDL number:

MFCD00036951

Beilstein/REAXYS Number:

4430959

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属性

product line

BioReagent

Quality Level

100

assay

≥95% (HPCE)

form

solid

mp

152-154 °C (lit.)

solubility

DMF: soluble, DMSO: soluble, acetonitrile: soluble, methanol: soluble

fluorescence

λ_ex 390 nm; λ_em 478 nm in 0.1 M phosphate pH 7.5 (after derivatization with glutathione), λ_ex 398 nm

suitability

suitable for fluorescence

storage temp.

2-8°C

SMILES string

CC1=C(C)C(=O)N2N1C(CBr)=C(C)C2=O

InChI

1S/C10H11BrN2O2/c1-5-7(3)12-8(4-11)6(2)10(15)13(12)9(5)14/h4H2,1-3H3

InChI key

AHEWZZJEDQVLOP-UHFFFAOYSA-N

说明

General description

溴乙烷也被称为硫醇探针，是一种通过光解反应激活的荧光试剂。溴乙烷在溶液中与小硫醇基团（比如 GSH）和有反应活性的蛋白硫醇基团（如血红蛋白）反应。溴乙烷与硫醇的反应是二级反应，和pH相关。并且，通过与硫醇反应，激活了用于检测的水溶性荧光物质。

Application

溴乙烷被用于通过HPLC测定硫醇。它适用于作为荧光测定2,3-二巯基丙烷-1-磺酸和其他二硫醇的柱前衍生试剂。溴乙烷被用做研究牛心脏线粒体中对寡霉素敏感的ATP酶的荧光标记。

Features and Benefits

用溴乙烷分析低分子量硫醇的好处在于：高选择性。

反应快速。
易于通过反相HPLC分离衍生物。
能穿透细胞。

Packaging

无底玻璃瓶。内含物在插入的融合锥体内。

Other Notes

荧光硫醇特异性试剂

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存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

历史批次信息供参考:

分析证书(COA)

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Determination of thiol proteins using monobromobimane labeling and high-performance liquid chromatographic analysis: application to Escherichia coli thioredoxin.

P C Chinn et al.

Analytical biochemistry, 159(1), 143-149 (1986-11-15)

A highly sensitive and specific assay for Escherichia coli thioredoxin was developed using the thiol-specific reagent monobromobimane. Treatment of dithiothreitol-reduced thioredoxin with an excess of monobromobimane in Tris buffer (pH 8.0, 23 degrees C) for 30 min resulted in the

Fluorometric determination of 2,3-dimercaptopropane-1-sulfonic acid and other dithiols by precolumn derivatization with bromobimane and column liquid chromatography.

R M Maiorino et al.

Journal of chromatography, 374(2), 297-310 (1986-01-24)

The increasing therapeutic use of dithiol metal binding agents, such as 2,3-dimercaptopropane-1-sulfonic acid (DMPS), has stimulated the need for a sensitive and selective method for their determination in biological fluids. A method has now been developed in which DMPS was

Short-distance probes for protein backbone structure based on energy transfer between bimane and transition metal ions.

Justin W Taraska et al.

Proceedings of the National Academy of Sciences of the United States of America, 106(38), 16227-16232 (2009-10-07)

The structure and dynamics of proteins underlies the workings of virtually every biological process. Existing biophysical methods are inadequate to measure protein structure at atomic resolution, on a rapid time scale, with limited amounts of protein, and in the context

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全球贸易项目编号

货号	GTIN
69898-100MG	04061833278086
69898-25MG	04061832794839