79390
磷酸酶(碱性) 来源于小牛肠粘膜
suitable for enzyme immunoassay, solution (clear, colorless), ~2500 U/mg protein (~10 mg/ml)
别名:
Alkaline Phosphatase from calf intestinal mucosa
登录 查看组织和合同定价。
选择尺寸
变更视图
关于此项目
化学文摘社编号:
UNSPSC Code:
12352204
eCl@ss:
42010105
EC Number:
232-631-4
NACRES:
NA.54
MDL number:
Specific activity:
~2500 U/mg protein (~10 mg/ml)
Biological source:
bovine (calf) intestinal mucosa
biological source
bovine (calf) intestinal mucosa
form
solution (clear, colorless)
specific activity
~2500 U/mg protein (~10 mg/ml)
technique(s)
enzyme immunoassay: suitable
impurities
0.1 mM ZnCl2, 1 mM MgCl2, 3 M NaCl, 30 mM triethanolamine
pH
~7.6
storage temp.
2-8°C
Application
Alkaline phosphatase is used in the laboratory to remove phosphate monoesters which prevents self-ligation. It is also used in the dairy industry to confirm pasturization of milk. Alkaline phosphatse is commonly conjugated to antibodies and used in immunoblotting, ELISA′s, and other antibody based detection assays.
Biochem/physiol Actions
Alkaline phosphatase is a hydrolytic enzyme which dephosphorylates a variety of molecules under basic conditions.
Preparation Note
The solution can be used without dialysis (for enzyme immuno-assay).
Other Notes
Used in highly sensitive (pg/m) enzyme immunoassays with LCEC detection of phenol; Comparison of three methods for conjugating alkaline phosphatase to human placental lactogen; Preparation of enzyme-antibody conjugates; Liposome immunoassay with antigen-coupled liposomes containing alkaline phosphatase
1 U corresponds to the amount of enzyme which hydrolyzes 1 μmol 4-nitrophenyl phosphate per minute at pH 9.8 and 37 °C
Sales restrictions may apply
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
低风险生物材料
动植物源性产品
此项目有
M.I. Halliday et al.
Biochemical Society Transactions, 14, 473-473 (1986)
D G Williams
Journal of immunological methods, 72(1), 261-268 (1984-08-03)
One of the requirements for enzyme immunoassay is the formation of a labelled component of the assay system which can be either the ligand or the binder. Three methods for conjugating alkaline phosphatase to human placental lactogen were investigated, involving
K.R. Wehrmeyer et al.
Journal of Liquid Chromatography, 6, 2141-2141 (1983)
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| 109606-250G | 04061838688699 |
| 8057920250 | 04022536387338 |
| 109606-500G | 04061838688750 |
| 79390-1MG | 04061832952062 |
| 79390-5MG | 04061832754437 |