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94403

Hoechst 33258 溶液

1 mg/mL in H2O, ≥98.0% (HPLC)

别名:

双苯并咪唑H 33258溶液, 赫斯特荧光染料 33258 溶液

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关于此项目

经验公式（希尔记法）:
C25H24N6O · 3HCl
化学文摘社编号:
23491-45-4
分子量:
533.88
MDL number:
MFCD29074011
UNSPSC Code:
12171500
PubChem Substance ID:
329770334
NACRES:
NA.32
Assay:
≥98.0% (HPLC)
Form:
liquid
Quality level:
100
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Quality Level

100

assay

≥98.0% (HPLC)

form

liquid

mol wt

533.88 g/mol

concentration

1 mg/mL in H2O

color

colorless, very light green-yellow to dark green-yellow, very light yellow to dark yellow

pH

6.0-8.0 (25 °C)

solubility

DMF: soluble, H2O: soluble

density

1.00 g/cm3

fluorescence

λex 355 nm; λem 465 nm in TE buffer; DNA

storage temp.

2-8°C

SMILES string

Cl.Cl.Cl.CN1CCN(CC1)c2ccc3[nH]c(nc3c2)-c4ccc5nc([nH]c5c4)-c6ccc(O)cc6

InChI

1S/C25H24N6O.3ClH/c1-30-10-12-31(13-11-30)18-5-9-21-23(15-18)29-25(27-21)17-4-8-20-22(14-17)28-24(26-20)16-2-6-19(32)7-3-16;;;/h2-9,14-15,32H,10-13H2,1H3,(H,26,28)(H,27,29);3*1H

InChI key

SMNPLAKEGAEPJD-UHFFFAOYSA-N

General description

赫斯特 33258，也被称为双联苯亚胺，是一种蓝色荧光染料。它被归类为一种染色体染剂，可与DNA双螺旋的小沟结合，识别一组四对A-T碱基对。

Application

赫斯特 33258被用于荧光法估算样品中DNA浓度，这种方法比分光光度法更好，它可以测定纳克级DNA。并且，由于赫斯特 33258适用于识别细胞培养基中的支原体污染，由于其高荧光性。

存储类别

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
BCCK6782
BCCH3038
BCCH1899
BCCG8111
BCCG4764

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货号GTIN
94403-1ML04061838069023