产品名称
过表达 ™ C41 (DE3) 化学感受态细胞, for the highest protein expression
biological source
Escherichia coli
grade
Molecular Biology
growth mode
adherent or suspension
morphology
rod shaped
technique(s)
microbiological culture: suitable
cell transformation
competent cell type: chemically competent
transformation efficiency: ≥1 x 106 cfu/μg
shipped in
dry ice
storage temp.
−70°C
General description
OverExpress™ 电感受态和化学感受态细胞是可以有效表达所有种类生物体（包括真细菌、酵母、植物、病毒和哺乳动物）的有毒蛋白质的大肠杆菌。这些新菌株表达有毒蛋白质的有效性已在350多篇论文中得到验证。OverExpress™菌株含有从表型上选择的基因突变，以产生对有毒蛋白质的耐受性。C41(DE3) 菌株来源于 BL21(DE3)。该菌株至少有一处突变，其防止了与许多重组有毒蛋白表达相关的细胞死亡。菌株C43（DE3）来源于C41（DE3），是通过选择对不同毒性蛋白的抗性而得到的，并且可以表达一组不同的C41（DE3）的毒性蛋白。OverExpress C41（DE3）pLysS和C43（DE3）pLysS还带有抗氯霉素的质粒，该质粒可编码T7溶菌酶，其是T7 RNA聚合酶的一种天然抑制剂。含有pLysS的细胞会产生少量的T7溶菌酶。这些菌株用于抑制诱导以前T7 RNA聚合酶的基础表达，从而使编码特别有毒的蛋白质的重组体保持稳定。
基因型
F – ompT hsdSB (rB- mB-) gal dcm (DE3)
基因型
F – ompT hsdSB (rB- mB-) gal dcm (DE3)
Application
过表达 ™ C41 (DE3) 化学感受态细胞已用于表达筛选。
Features and Benefits
- 用这些 BL21 (DE3) 衍生物克隆到任何 T7 载体的表达基因
- 有效表示毒性和膜蛋白类
- 被超过 350 篇研究文章引用
Other Notes
- 过表达 C41 (DE3) 化学感受态细胞
- pUC 19 转化对照 DNA
- 表达回收培养基
Legal Information
OverExpress is a trademark of Imaxio, S.A.
存储类别
10 - Combustible liquids
wgk
WGK 2
法规信息
高风险级别生物产品--病毒，疫苗等
此项目有
实验方案
Protocol for OverExpress™ Chemically Competent Cells. Product Numbers: CMC0017, CMC0018, CMC0019, CMC0020, CMC0023, CMC0024
A high-throughput expression screening platform to optimize the production of antimicrobial peptides.
Schreiber C, et al.
Microbial cell factories, 16(1), 29-29 (2017)
Myles B C Dillon et al.
ACS chemical biology, 7(7), 1198-1204 (2012-04-18)
Protein arginine methyltransferases (PRMTs) catalyze the posttranslational methylation of arginine using S-adenosylmethionine (SAM) as a methyl-donor. The PRMT family is widely expressed and has been implicated in biological functions such as RNA splicing, transcriptional control, signal transduction, and DNA repair.
Ji-Young Youn et al.
Molecular biology of the cell, 21(17), 3054-3069 (2010-07-09)
BAR domains are protein modules that bind to membranes and promote membrane curvature. One type of BAR domain, the N-BAR domain, contains an additional N-terminal amphipathic helix, which contributes to membrane-binding and bending activities. The only known N-BAR-domain proteins in
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| CMC0017-20X40UL | 04061838042477 |