grade
Molecular Biology
sterility
non-sterile
form
powder
composition
NaCl, 5 g/L , Tryptone, 10 g/L , Yeast Extract, 5 g/L
technique(s)
microbiological culture: suitable
pH
6.8-7.2(2% solution)
application(s)
food and beverages
microbiology
storage temp.
room temp
suitability
nonselective for Escherichia coli, nonselective for coliforms
General description
Lennox LB是一个被高度引用的用于 大肠杆菌培养的微生物生长培养基。这种富含营养的微生物肉汤含有肽、氨基酸和低盐配方的碳水化合物。
Application
Luria肉汤被用于大肠杆菌(Escherichia coli)的维持和繁殖。 大肠杆菌在Luria肉汤中生长得更快,因为胰蛋白胨和酵母为其提供了必需的生长因子,从而无需大肠杆菌自身合成。由于含有NaCl成分,Luria肉汤还含有转运和渗透平衡所必需的电解质。
在三鞭毛虫细胞提取物的分级研究中,它被用于梨形四膜虫胞级分的重悬。
在三鞭毛虫细胞提取物的分级研究中,它被用于梨形四膜虫胞级分的重悬。
适用于 大肠杆菌 菌株的非选择性培养,用于克隆、生产DNA质粒和生产重组蛋白。 也适用于添加适当抗生素时的选择性培养,包括需要低盐条件的抗生素,如吉欧霉素®。
Features and Benefits
Lennox LB粉末可提供:
- 便捷式小包装,无需称重步骤
- 使用大包装可轻松放大规模
- 一种预算较低的液体替代品
- 标准配方
Preparation Note
1.将20 g悬浮于1L蒸馏水中。
2.121 °C下高压灭菌15分钟。
制备LB肉汤:添加1 g葡萄糖,然后按照上述说明操作。
制备Enquist &Sternberg培养基:高压蒸汽灭菌后,以无菌操作加入10 mL无菌1M硫酸镁。
2.121 °C下高压灭菌15分钟。
制备LB肉汤:添加1 g葡萄糖,然后按照上述说明操作。
制备Enquist &Sternberg培养基:高压蒸汽灭菌后,以无菌操作加入10 mL无菌1M硫酸镁。
Legal Information
Zeocin is a registered trademark of Cayla Sarl
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存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
监管及禁止进口产品
此项目有
实验方案
在从小型培养到发酵级的不同规模下培养大肠杆菌。包括了用于启动起始培养物、悬浮细胞、单一培养物和平板噬菌体M13的实验方案。
General protocols for growth of competent cells in microbial medium.
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Frontiers in microbiology, 8, 1152-1152 (2017-07-12)
Quorum sensing (QS) is a density-dependent mechanism allowing bacteria to synchronize their physiological activities, mediated by a wide range of signaling molecules including
J Benavente et al.
International journal of biological macromolecules, 103, 758-763 (2017-05-27)
The preparation of silver nanoparticles (AgNPs) and their incorporation into the structure of a regenerated cellulose membrane by dip coating is presented. Morphological characterization of the AgNPs (average diameter of 20±2nm) was carried out by SEM/TEM, while elastic, electrical and
Cheyann Lee Wetteland et al.
Acta biomaterialia, 35, 341-356 (2016-03-01)
This article reports the quantitative relationship between the concentration of magnesium oxide (MgO) nanoparticles and its distinct biological activities towards mammalian cells and infectious bacteria for the first time. The effects of MgO nanoparticles on the viability of bone marrow
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| L3022-6X1KG | 04061835573448 |
| L3022-1KG | 04061835573424 |
| L3022-250G | 04061835573431 |