产品名称
磷酸盐缓冲溶液, 10× PBS for Western blots and IP
grade
Molecular Biology
Quality Level
sterility
0.2 μm filtered
form
liquid
technique(s)
immunoprecipitation (IP): suitable, western blot: suitable
pH
7.2-7.6(1x solution)
storage temp.
room temp
General description
磷酸盐缓冲盐水（PBS）通常用作蛋白质印迹和免疫沉淀（IP）程序中的洗涤缓冲液，以及用于一般分子生物学应用的细胞裂解物的样品制备。
Application
适用于蛋白质印迹和IP的洗涤缓冲液，以及细胞裂解液样品制备。
Preparation Note
10x PBS 含有：
- 30mM 磷酸钠
- 1.5M 氯化钠
- 10.5mM 磷酸钾
使用分子生物级水将 10x PBS 稀释至 1x工作溶液。
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存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
商品
Learn how water impurities can affect pH. Water quality defined as pure water or Type 2 water is recommended to prepare buffer solutions or measure pH.
Johan E Karlsson et al.
Drug metabolism and disposition: the biological fate of chemicals, 38(4), 705-714 (2010-01-15)
Membrane-bound transporter proteins play an important role in the efflux of drugs from cells and can significantly influence the pharmacokinetics of drug molecules. This study describes the production of large amounts of high-activity transporter membrane vesicles from human embryonic kidney
Caroline R Archer et al.
Scientific reports, 8(1), 10160-10160 (2018-07-07)
Pharmaceutical agents despite their efficacy to treat disease can cause additional unwanted cardiovascular side effects. Cardiotoxicity is characterized by changes in either the function and/or structure of the myocardium. Over recent years, functional cardiotoxicity has received much attention, however morphological
Jennifer L S Sporty et al.
Analytical chemistry, 82(15), 6593-6600 (2010-07-14)
A novel method for extracting butyrylcholinesterase (BuChE) from serum as a means of identifying and measuring nerve agent adducts to human BuChE is presented here. Antibutyrylcholinesterase monoclonal antibodies were conjugated to protein-G ferromagnetic particles and mixed with 500 microL serum
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| P7059-1L | 04061834394297 |