跳转至内容
Merck
CN

B6429

酪蛋白封闭缓冲液 10x

for Northern and Southern blotting, solution

别名:

10x Blocking Buffer

登录 查看组织和合同定价。

选择尺寸

变更视图

关于此项目

NACRES:
NA.54
UNSPSC Code:
12161700
技术服务
需要帮助?我们经验丰富的科学家团队随时乐意为您服务。
让我们为您提供帮助


grade

Molecular Biology

Quality Level

form

solution

technique(s)

Southern blotting: suitable

storage temp.

room temp

General description

酪蛋白是存在于乳汁中的磷蛋白。它分为四种亚型,包括αS1-、αS2-、β-和 κ-酪蛋白。
Sigma 的 10x 封闭缓冲液是一种基于酪蛋白的封闭剂,常规用于减少检测试剂与 Northern 和 Southern 印迹膜的非特异性结合。

Application

酪蛋白封闭缓冲液10X已用于:

  • 蛋白印迹
  • 酶联免疫吸附分析(ELISA)
  • 免疫组化

Features and Benefits

  • 封闭性能优于奶粉或 BSA
  • 易于使用的浓缩液
  • 与中性和正电荷膜兼容
  • 与化学发光和比色检测试剂盒兼容

Preparation Note

使用前用分子生物学级水稀释 10x 封闭缓冲液。


pictograms

Exclamation markEnvironment

signalword

Warning

Hazard Classifications

Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

存储类别

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

低风险生物材料

此项目有



历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

没有发现合适的版本?

如果您需要特殊版本,可通过批号或批次号查找具体证书。

已有该产品?

在文件库中查找您最近购买产品的文档。

访问文档库


实验方案

Northern和Southern印迹简介:两种将大分子转移到膜支持物的常用方法。该文章还提供了Northern印迹和Southern印迹的实验方案。

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

Protocol for Casein Blocking Buffer in Southern and Western blotting includes troubleshooting for optimizing signal.


Katarzyna Z Haza et al.
Nature communications, 12(1), 4045-4045 (2021-07-02)
RAS mutations are the most common oncogenic drivers across human cancers, but there remains a paucity of clinically-validated pharmacological inhibitors of RAS, as druggable pockets have proven difficult to identify. Here, we identify two RAS-binding Affimer proteins, K3 and K6
Michelle M Adams et al.
Experimental neurology, 211(1), 141-149 (2008-03-18)
Caloric restriction (CR) is a daily reduction of total caloric intake without a decrease in micronutrients or disproportionate reduction of any one dietary component. CR can increase lifespan reliably in a wide range of species and appears to counteract some
Princy Parsana et al.
BMC cancer, 17(1), 447-447 (2017-06-28)
Epithelial to mesenchymal transition (EMT) is the process by which stationary epithelial cells transdifferentiate to mesenchymal cells with increased motility. EMT is integral in early stages of development and wound healing. Studies have shown that EMT could be a critical



全球贸易项目编号

货号GTIN
B6429-500ML04061833434796