grade
Molecular Biology
Quality Level
form
solution
technique(s)
Southern blotting: suitable
storage temp.
room temp
General description
酪蛋白是存在于乳汁中的磷蛋白。它分为四种亚型,包括αS1-、αS2-、β-和 κ-酪蛋白。
Sigma 的 10x 封闭缓冲液是一种基于酪蛋白的封闭剂,常规用于减少检测试剂与 Northern 和 Southern 印迹膜的非特异性结合。
Sigma 的 10x 封闭缓冲液是一种基于酪蛋白的封闭剂,常规用于减少检测试剂与 Northern 和 Southern 印迹膜的非特异性结合。
Application
酪蛋白封闭缓冲液10X已用于:
- 蛋白印迹
- 酶联免疫吸附分析(ELISA)
- 免疫组化
Features and Benefits
- 封闭性能优于奶粉或 BSA
- 易于使用的浓缩液
- 与中性和正电荷膜兼容
- 与化学发光和比色检测试剂盒兼容
Preparation Note
使用前用分子生物学级水稀释 10x 封闭缓冲液。
signalword
Warning
hcodes
Hazard Classifications
Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1
存储类别
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
低风险生物材料
此项目有
实验方案
Northern和Southern印迹简介:两种将大分子转移到膜支持物的常用方法。该文章还提供了Northern印迹和Southern印迹的实验方案。
Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.
Protocol for Casein Blocking Buffer in Southern and Western blotting includes troubleshooting for optimizing signal.
Katarzyna Z Haza et al.
Nature communications, 12(1), 4045-4045 (2021-07-02)
RAS mutations are the most common oncogenic drivers across human cancers, but there remains a paucity of clinically-validated pharmacological inhibitors of RAS, as druggable pockets have proven difficult to identify. Here, we identify two RAS-binding Affimer proteins, K3 and K6
Michelle M Adams et al.
Experimental neurology, 211(1), 141-149 (2008-03-18)
Caloric restriction (CR) is a daily reduction of total caloric intake without a decrease in micronutrients or disproportionate reduction of any one dietary component. CR can increase lifespan reliably in a wide range of species and appears to counteract some
Princy Parsana et al.
BMC cancer, 17(1), 447-447 (2017-06-28)
Epithelial to mesenchymal transition (EMT) is the process by which stationary epithelial cells transdifferentiate to mesenchymal cells with increased motility. EMT is integral in early stages of development and wound healing. Studies have shown that EMT could be a critical
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| B6429-500ML | 04061833434796 |

