H7033
PerfectHyb™ Plus杂交缓冲液
for Northern and Southern blotting, solution
别名:
hyb buffer, hyb solution, hybridization solution, northern hybridization buffer, southern hybridization buffer
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关于此项目
NACRES:
NA.25
UNSPSC Code:
12161700
General description
Sigma的PerfectHyb Plus是一种即用型杂交缓冲液，适用于Northern和Southern印迹方案。 使用PerfectHyb Plus可将杂交时间缩短至两小时。
Application
可用作Northern印迹、Southern印迹、菌落/噬斑印迹和斑点印迹中所使用的杂交缓冲液。
Features and Benefits
- 快速且易于使用
- 灵敏度比传统缓冲液高20倍
- 不需要封闭步骤
- 与中性和带正电的膜兼容
- 与化学发光、比色和DIG检测分析兼容
- 兼容放射性和非放射性探针
- 与单链或双链DNA、RNA和寡核苷酸探针兼容
Other Notes
在溶液中形成的少量沉淀物可以在30-35℃下，通过偶尔轻缓颠倒进行溶解。
Legal Information
PerfectHyb is a trademark of Sigma-Aldrich Co. LLC
存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
实验方案
Northern和Southern印迹简介：两种将大分子转移到膜支持物的常用方法。该文章还提供了Northern印迹和Southern印迹的实验方案。
Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.
The miRNA pathway limits AGO1 availability during siRNA-mediated PTGS defense against exogenous RNA.
Angel Emilio Martínez de Alba et al.
Nucleic acids research, 39(21), 9339-9344 (2011-08-05)
In plants, most microRNAs (miRNAs) and several endogenous small interfering RNAs (siRNAs) bind to ARGONAUTE1 (AGO1) to regulate the expression of endogenous genes through post-transcriptional gene silencing (PTGS). AGO1 also participates in a siRNA-mediated PTGS defense response that thwarts exogenous
Tao Qin et al.
Plant physiology, 175(3), 1321-1336 (2017-09-10)
Long noncoding RNAs (lncRNAs) affect gene expression through a wide range of mechanisms and are considered as important regulators in many essential biological processes. A large number of lncRNA transcripts have been predicted or identified in plants in recent years.
Sivaramakrishnan Muthuswamy et al.
Planta, 233(1), 201-208 (2010-09-28)
Protein sumoylation plays an important role in plant development, flowering-time regulation, and abiotic stress response. However, the molecular role of sumoylation in these pathways is largely unknown. It was shown previously that in mutants of the inner nuclear basket nucleoporin
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| H7033-125ML | 04061833803080 |
| H7033-50ML | 04061833803165 |
| H7033-1L | 04061833803097 |