P8849
Protease Inhibitor Cocktail
DMSO solution, for the inhibition of serine, cysteine, aspartic, aminopeptidases and thermolysin-like activities, for use in purification of Histidine-tagged proteins, DMSO solution
别名:
蛋白酶抑制剂溶液
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关于此项目
Form:
DMSO solution
Solubility:
water: soluble
Storage temp.:
−20°C
产品名称
Protease Inhibitor Cocktail, for use in purification of Histidine-tagged proteins, DMSO solution
biological source
synthetic
Quality Level
form
DMSO solution
solubility
water: soluble
storage temp.
−20°C
General description
蛋白酶抑制剂的DMSO溶液专用于纯化组氨酸标签蛋白。
溶液中混有多种抑制剂成分，可特异性抑制丝氨酸、半胱氨酸、天冬氨酸、嗜热菌蛋白酶样蛋白酶和氨基肽酶。
溶液中混有多种抑制剂成分，可特异性抑制丝氨酸、半胱氨酸、天冬氨酸、嗜热菌蛋白酶样蛋白酶和氨基肽酶。
Application
混合液经过专门的优化和验证，适合分离组氨酸标签(His-tag)蛋白质，特意去除螯合剂后尤其适合用于固定化金属亲和层析(IMAC)分离。
推荐用量：1 mL溶液抑制20g大肠杆菌细胞或10g杆状病毒感染草地夜蛾蛹卵巢细胞得到的100ml细胞裂解液中的蛋白酶活性。
推荐用量：1 mL溶液抑制20g大肠杆菌细胞或10g杆状病毒感染草地夜蛾蛹卵巢细胞得到的100ml细胞裂解液中的蛋白酶活性。
Biochem/physiol Actions
抑制丝氨酸，半胱氨酸，天冬氨酸，和嗜热菌蛋白酶样蛋白酶和氨基肽酶。
Features and Benefits
专门为组氨酸标签蛋白纯化而配制。
靶向多种蛋白酶，具有全面的蛋白酶抑制活性。
不加螯合剂，适合IMAC应用。
方便的包装选择。
靶向多种蛋白酶，具有全面的蛋白酶抑制活性。
不加螯合剂，适合IMAC应用。
方便的包装选择。
Preparation Note
推荐采用1mL抑制从20gEscherichia coli或10g杆状病毒感染 Spodoptera frugiperda蛹卵巢细胞提取的总体积100ml的蛋白酶。
该蛋白酶抑制剂混合物已针对组蛋白标记蛋白进行了优化和测试
该蛋白酶抑制剂混合物已针对组蛋白标记蛋白进行了优化和测试
该产品在DMSO中是澄清溶液。 推荐1mL溶液可以抑制20gE. Coli细胞或10g细胞或10g 杆状病毒感染细胞得到的100ml细胞裂解液中的蛋白酶活性。
Other Notes
AEBSF
乌苯美司
E-64
Pepstatin A
磷阿米酮
乌苯美司
E-64
Pepstatin A
磷阿米酮
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存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
185.0 °F - closed cup
flash_point_c
85 °C - closed cup
法规信息
常规特殊物品
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全球贸易项目编号
| 货号 | GTIN |
|---|---|
| P8849-1ML | 04061834401933 |
| P8849-5ML | 04061838446558 |