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Merck
CN

PP0201

Glycoprofile II, Enzymatic In-Solution N-Deglycosylation Kit

sufficient for 20 samples

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关于此项目

NACRES:
NA.26
UNSPSC Code:
12352202
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Quality Level

storage temp.

2-8°C

General description

含有足够进行至少20次反应所需的试剂,对于典型的糖蛋白,可处理样品范围为1~2 mg。

Application

Glycoprofile II, Enzymatic In-Solution N-去糖基化试剂盒已用于裂褶菌凝集素、拟南芥甘露聚糖酶、人脐静脉内皮细胞(HUVEC)蛋白裂解物和有机溶质转运蛋白Ostβ的去糖基化。
GlycoProfile II Enzymatic In-Solution N-去糖基化试剂盒用于研究裂褶菌中N-乙酰-D-半乳糖胺(GalNAc)特异性凝集素的糖基化作用。
GlycoProfile II经过优化,可提供一种方便且可重复的从糖蛋白中去除N-连接聚糖的方法,与后续的MALDI-TOF质谱分析兼容,而不受任何反应成分的干扰。

Legal Information

GlycoProfile is a trademark of Sigma-Aldrich Co. LLC


仅试剂盒组分

产品编号
说明

  • 10× Reaction Buffer 1 mL/vial

试剂盒组分也可单独购买

产品编号
说明
化学品安全说明书 & 价格

  • PNGase F from Elizabethkingia meningoseptica, BioReagent, ≥95% (SDS-PAGE) 50 U
    化学品安全说明书

  • Ribonuclease B from bovine pancreas, BioReagent, ≥50 Kunitz units/mg protein, ≥80% (SDS-PAGE) .5 mg
    化学品安全说明书

  • Octyl β-D-glucopyranoside, BioXtra, ≥98% (GC) 100 mg
    化学品安全说明书

  • 2-Mercaptoethanol, Molecular Biology, suitable for electrophoresis, suitable for cell culture, BioReagent, 99% (GC/titration) .9 mL
    化学品安全说明书

signalword

Danger

Hazard Classifications

Acute Tox. 2 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 2 Oral

target_organs

Liver,Heart

存储类别

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

wgk

WGK 3

法规信息

低风险生物材料
危险化学品
常规特殊物品

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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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实验方案

Explore mass spectrometry analysis of glycans for glycomic & glycoproteomic neutral & acidic glycan analysis. See a general mass spec glycan analysis procedure.

商品

Find deglycosylation kits for your research. Review the different features of deglycosylation kits for glycoproteins, enzymatic proteins, and native proteins.


Enzymatic characterization of a glycoside hydrolase family 5 subfamily 7 (GH5\_7) mannanase from Arabidopsis thaliana
Wang Y, et al.
Planta, 239(3), 653-665 (2014)
Christen Y L Yuen et al.
Plant & cell physiology, 58(6), 1103-1117 (2017-04-27)
Members of the protein disulfide isomerase (PDI)-C subfamily are chimeric proteins containing the thioredoxin (Trx) domain of PDIs, and the conserved N- and C-terminal Pfam domains of Erv41p/Erv46p-type cargo receptors. They are unique to plants and chromalveolates. The Arabidopsis genome
beta-Subunit of the Ostalpha-Ostbeta organic solute transporter is required not only for heterodimerization and trafficking but also for function
Christian WV, et al.
Test, 287(25), 21233-21243 (2012)



全球贸易项目编号

货号GTIN
PP0201-1KT04061836686895