R7884
核糖核酸酶B 来源于牛胰腺
BioReagent, ≥50 Kunitz units/mg protein, ≥80% (SDS-PAGE)
别名:
RNase B
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关于此项目
化学文摘社编号:
UNSPSC Code:
12352204
EC Number:
232-646-6
NACRES:
NA.32
MDL number:
Specific activity:
≥50 Kunitz units/mg protein
Assay:
≥80% (SDS-PAGE)
Biological source:
bovine pancreas
Concentration:
≥60%
Quality Level
biological source
bovine pancreas
product line
BioReagent
assay
≥80% (SDS-PAGE)
form
powder
specific activity
≥50 Kunitz units/mg protein
concentration
≥60%
technique(s)
cell based assay: suitable
suitability
suitable for molecular biology
application(s)
cell analysis
foreign activity
protease ≤0.001 units/mg solid
storage temp.
−20°C
SMILES string
[nH]1cncc1CC(NC(=O)CCN)C(=O)O
InChI
1S/C9H14N4O3/c10-2-1-8(14)13-7(9(15)16)3-6-4-11-5-12-6/h4-5,7H,1-3,10H2,(H,11,12)(H,13,14)(H,15,16)
InChI key
CQOVPNPJLQNMDC-UHFFFAOYSA-N
Application
在细胞周期平台分析期间，牛胰腺核糖核酸酶B可用于消化RNA。
Biochem/physiol Actions
通过枯草杆菌蛋白酶消化天然RNase B（包含RNase B的氨基酸残基21-124）产生的天然RNase BS对PNGase F消化敏感。 牛胰腺RNase B的分子内N-聚糖具有伴侣蛋白的作用。 已有研究发现RNase B比RNase A起效更快，而RNase A在再生过程中易于聚集。Asn-寡糖（对应于RNase B的最主要糖链）的刺激作用表面RNase B的N-聚糖可促进大体积中间体转化为折叠的紧密物种。
Packaging
包装尺寸取决于蛋白质含量
Preparation Note
通过亲和层析纯化
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
低风险生物材料
此项目有
商品
PNGase Fast denaturing buffer and enzyme provide results similar to a conventional 20-hour protocol, reducing workflow time to about 1 hour.
Véronique Blanchard et al.
Biochemistry, 47(11), 3435-3446 (2008-02-26)
In glycoanalysis protocols, N-glycans from glycoproteins are most frequently released with peptide- N (4)-( N-acetyl-beta-glucosaminyl)asparagine amidase F (PNGase F). As the enzyme is an amidase, it cleaves the NH-CO linkage between the Asn side chain and the Asn-bound GlcNAc residue.
H Yamaguchi et al.
Journal of biochemistry, 120(3), 474-477 (1996-09-01)
This paper describes a chaperone-like function of the intramolecular N-glycans of bovine pancreatic RNase B. We studied air-oxidative regeneration from reductively denatured species of RNase B and its nonglycosylated form, RNase A. RNase B was reactivated much faster than RNase
Audra A Hargett et al.
Molecules (Basel, Switzerland), 26(14) (2021-07-25)
Protein glycosylation is important in many organisms for proper protein folding, signaling, cell adhesion, protein-protein interactions, and immune responses. Thus, effectively determining the extent of glycosylation in glycoprotein therapeutics is crucial. Up to now, characterizing protein glycosylation has been carried
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| R7884-500UG | 04061836807344 |
| R7884-100MG | 04061836801892 |
| R7884-5MG | 04061833290323 |