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Merck
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STRN50

Spectrum 植物总RNA试剂盒

greener alternative

sufficient for 50 purifications

别名:

Plant total RNA extraction kit, Plant total RNA isolation kit, Plant total RNA purification kit

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关于此项目

NACRES:
NA.52
UNSPSC Code:
41105501
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usage

sufficient for 50 purifications

Quality Level

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

technique(s)

RNA purification: suitable

test parameters

: ~30 min kit run time, sample volume: 100 mg

greener alternative category

storage temp.

15-25°C

General description

Spectrum植物总RNA试剂盒利用了裂解液、结合化学反应法以及方便的柱式“结合-洗涤-洗脱”形式,可在大约30分钟内从100 mg组织中纯化出多达100 μg总RNA。典型得率范围为20–60 μg。

在液氮中将组织研磨成细粉末之后,细胞裂解,细胞残渣通过物理和化学方法与内源性RNA分离。随后,RNA结合到柱式支撑硅胶底物上,经过几遍洗涤去除污染物。

从柱上洗脱总RNA,然后在Northern杂交、RT-PCR和qRT-PCR等常规应用中使用。
我们致力于为您提供更环保的替代产品,以符合“绿色化学的12项原则”的一项或多项原则要求。本产品含有本安型化学制剂。

Application

光谱 植物总RNA试剂盒已被用于从培养的棉纤维的地纤维组织、印度楝组织、以及 瓶子草(一种食虫植物)中分离RNA。

Features and Benefits

  • 每次制备至多可获得60 μg高纯度浓缩RNA
  • 实验方案高度有效,可在30分钟内完成RNA纯化
  • 专为研究困难的植物组织而设计

Legal Information

Spectrum is a trademark of Sigma-Aldrich Co. LLC


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仅试剂盒组分

产品编号
说明

  • Binding Solution

  • Lysis Solution

  • Wash Solution 1

试剂盒组分也可单独购买

产品编号
说明
化学品安全说明书 & 价格

  • 2-Mercaptoethanol, Molecular Biology, suitable for electrophoresis, suitable for cell culture, BioReagent, 99% (GC/titration)
    化学品安全说明书

signalword

Danger

Hazard Classifications

Acute Tox. 2 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 2 Oral

target_organs

Liver,Heart

存储类别

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

wgk

WGK 3

法规信息

危险化学品

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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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商品

简单的DNA和RNA纯法方法大幅推动了基因组和基因表达的分析和鉴定。人们需要快速、方便地从多种细胞来源分离DNA和RNA,包括哺乳动物、植物和细菌培养物来源的细胞和组织。

Simple DNA/RNA purification methods aid genome analysis from various sources, enhancing research efficiency.

相关内容

The Spectrum Plant Total RNA Kit incorporates a new, patent-pending lysis and binding chemistry. This kit is intended to purify total RNA from species and tissue where most Total RNA protocols fail. Without sacrificing affordability, you can purify more total RNA per isolation.


Transcriptome analysis of sarracenia, an insectivorous plant.
Srivastava A
DNA Research : An International Journal for Rapid Publication of Reports on Genes and Genomes, 18(4), 253-261 (2011)
Soybean DICER-LIKE2 Regulates Seed Coat Color via Production of Primary 22-Nucleotide Small Interfering RNAs from Long Inverted Repeats.
Jia, et al.
Plant Cell, 32, 3662-3673 (2021)
Gil Wiseglass et al.
Scientific reports, 9(1), 6476-6476 (2019-04-26)
Obligate root holoparasite Phelipanche aegyptiaca is an agricultural pest, which infests its hosts and feeds on the sap, subsequently damaging crop yield and quality. Its notoriously viable seed bank may serve as an ideal pest control target. The phytohormone abscisic



全球贸易项目编号

货号GTIN
STRN50-1KT04061835575008