grade
Molecular Biology
Quality Level
description
TB; quick dissolve, dust-free formulation
sterility
non-sterile
form
powder
technique(s)
microbiological culture: suitable
application(s)
agriculture
food and beverages
microbiology
storage temp.
room temp
suitability
nonselective for Escherichia coli, nonselective for coliforms
General description
Terrific Broth是一个被高度引用的用于大肠杆菌培养的微生物生长培养基。 这种营养丰富的微生物肉汤含有甘油、肽、氨基酸、水溶性维生素和碳水化合物。
Application
适用于大肠杆菌菌株的非选择性培养，用于克隆、高产量生产 DNA 质粒和高产量生产重组蛋白。当添加适当的抗生素时，也适用于选择性培养。
使用了很棒的肉汤：
使用了很棒的肉汤：
- 作为在 pET28GST-LIC 中选择性生长表达 MLL SET（核原癌基因）结构域的大肠杆菌 BL21（DE3） V2RpRARE 的培养基
- 培养含有表达 IL-8（interleukin-8） 质粒的 BL21（DE3） 细胞
- 很棒的肉汤也适用于细菌悬浮液（枯草芽孢杆菌菌株 1085B）
Features and Benefits
很棒的 Broth EZMix 粉末提供：
- 无尘颗粒，便于安全操作和更快混合
- 一种预算较低的液体替代品
- 改良配方（不含甘油）
- 可通过大包装轻松放大规模
Preparation Note
将 24.1 g 粉末和 4 mL 甘油悬浮在足量的去离子水中，制成 500 mL 溶液。 在121 °C下高温灭菌15分钟以灭菌。 先冷却再添加其他成分，如抗生素（如果需要）。
Other Notes
*请注意，某些产品规格以液体形式提供。 本品为粉末形式，并且这些规格反映了最终的重悬体积。
23.6g/L酵母提取物
11.8g/L 胰蛋白胨
9.4g/L K2HPO4
2.2g/L KH2PO4
23.6g/L酵母提取物
11.8g/L 胰蛋白胨
9.4g/L K2HPO4
2.2g/L KH2PO4
Legal Information
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存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
监管及禁止进口产品
此项目有
实验方案
在从小型培养到发酵级的不同规模下培养大肠杆菌。包括了用于启动起始培养物、悬浮细胞、单一培养物和平板噬菌体M13的实验方案。
General protocols for growth of competent cells in microbial medium.
Eve Toulza et al.
Applied and environmental microbiology, 76(13), 4521-4529 (2010-05-04)
Understanding the conditions leading to harmful algal blooms, especially those produced by toxic dinoflagellate species, is important for environmental and health safety. In addition to investigations into the environmental conditions necessary for the formation of toxic blooms, we postulate that
Idan Tuval et al.
Proceedings of the National Academy of Sciences of the United States of America, 102(7), 2277-2282 (2005-02-09)
Aerobic bacteria often live in thin fluid layers near solid-air-water contact lines, in which the biology of chemotaxis, metabolism, and cell-cell signaling is intimately connected to the physics of buoyancy, diffusion, and mixing. Using the geometry of a sessile drop
Paul Peluso et al.
Analytical biochemistry, 312(2), 113-124 (2003-01-18)
Antibody microarrays have the potential to revolutionize protein expression profiling. The intensity of specific signal produced on a feature of such an array is related to the amount of analyte that is captured from the biological mixture by the immobilized
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| T9179-1KG | 04061837380754 |
| T9179-2X5L | 04061835575053 |
| T9179-6X500ML | 04061837380785 |