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A0410

氨基烯丙基-dUTP 钠盐

Name: 氨基烯丙基-dUTP 钠盐
Brand: SIGMA

≥85%, lyophilized powder

别名:

5-[3-氨基烯丙基]-2′-脱氧尿苷 5′-三磷酸盐, AA-dUTP

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关于此项目

经验公式（希尔记法）:

C₁₂H₂₀N₃O₁₄P₃ · xNa⁺

化学文摘社编号:

936327-10-5

分子量:

523.22 (free acid basis)

MDL number:

MFCD06200396

UNSPSC Code:

41106305

NACRES:

NA.51

Assay:

≥85%

Form:

lyophilized powder

Storage temp.:

−70°C

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属性

产品名称

氨基烯丙基-dUTP 钠盐, ≥85%, lyophilized powder

Quality Level

200

assay

≥85%

form

lyophilized powder

shipped in

dry ice

storage temp.

−70°C

SMILES string

[P](=O)(O[P](=O)(OCC1OC(CC1O)N2C=C(C(=O)NC2=O)\C=C\CN)O)(O[P](=O)(O)O)O.N

InChI key

YWKTUHBZWBRYHX-TYYBGVCCSA-N

说明

Application

氨基烯丙基-dUTP钠盐已被用于：

添加至脱氧核苷三磷酸混合物中用于互补DNA（cDNA）的合成
生成染色质免疫沉淀（ChIP）探针
在RNA的逆转录中用于合成荧光标记的探针

氨基烯丙基-dUTP可用于通过聚合酶链式反应（PCR），在第一链cDNA的合成过程中进行间接稳定标记。氨基烯丙基-dUTP可用作反应性位点，用于后续的反应性荧光探针的标记。氨基烯丙基-dUTP也可用于构建反应性DNA支架。

Biochem/physiol Actions

氨基烯丙基-dUTP钠盐/5-（3-氨基烯丙基）-2′-脱氧尿苷5′-三磷酸（aa-dUTP）主要用于合成核酸杂交探针的两步标记法中。

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Fluorescent DNA hybridization probe preparation using amine modification and reactive dye coupling.

W Gregory Cox et al.

BioTechniques, 36(1), 114-122 (2004-01-27)

Fluorescent nucleic acid hybridization probes traditionally have been generated by enzymatic incorporation of dye-labeled nucleotides, even though incorporation efficiency is low and variable from dye to dye. Alternatively, 5-(3-aminoallyl)-2'-deoxyuridine 5'-triphosphate (aa-dUTP) is enzymatically incorporated to generate amine-modified DNA, which is

Probe production for in situ hybridization by PCR and subsequent covalent labeling with fluorescent dyes.

Dirsch O, Ji Y, Bohr J, et al.

Appl. Immunohistochem., 15, 332-337 (2007)

Identification of the differential expressive tumor associated genes in rectal cancers by cDNA microarray.

Xue-Qin Gao et al.

World journal of gastroenterology, 13(3), 341-348 (2007-01-19)

To identify tumor associated genes of rectal cancer and to probe the application possibility of gene expression profiles for the classification of tumors. Rectal cancer tissues and their paired normal mucosa were obtained from patients undergoing surgical resection of rectal

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