A1418
抗 小鼠 IgG（Fc 特异性）-碱性磷酸酶 山羊抗
affinity isolated antibody, buffered aqueous solution
别名:
Goat Anti-Mouse IgG (Fc specific)–AP
登录 查看组织和合同定价。
选择尺寸
变更视图
关于此项目
Conjugate:
alkaline phosphatase conjugate
Clone:
polyclonal
Application:
ELISA (d), IHC (p), WB
Citations:
24
biological source
goat
Quality Level
recombinant
expressed in goat
conjugate
alkaline phosphatase conjugate
antibody form
affinity isolated antibody
antibody product type
secondary antibodies
clone
polyclonal
form
buffered aqueous solution
species reactivity
mouse
should not react with
human
technique(s)
direct ELISA: 1:40,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:25, western blot: 1:50,000-1:100,000 using total cell extract of HeLa cells
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
General description
IgG 抗体在补体激活、吞噬作用、胎盘转运和细胞表面受体结合等体液免疫应答中起关键作用。抗小鼠 IgG（Fc 特异性）-碱性磷酸酶抗体（以1：10,000 的稀释度采用PBS-Tween 稀释）可用于 ELISA。它也可用于免疫印迹。山羊抗小鼠 IgG（Fc 特异性）-碱性磷酸酶抗体与小鼠 IgG Fc 片段、IgG 及其亚类 IgG1、IgG2a、IgG2b 和 IgG3 发生特异性反应，但不与人 IgG 和小鼠 IgA 或 IgM 发生反应。
Immunogen
纯化的小鼠 IgG Fc 片段。
Application
抗小鼠 IgG（Fc 特异性）-碱性磷酸酶抗体可用于 ELISPOT（免疫斑点）测定。它也可用于免疫组化和蛋白质印迹。
碱性磷酸酶缀合的山羊抗小鼠 Fc 特异性抗体在 ELISA 测定中用作二抗，在 PBS/0.1% Tween 和 1% BSA中以 1:1000 的稀释度，在 37°C 下持续 1.5 小时。该抗体经开发使用磷酸 4-硝基苯酯（Sigma）作为底物，在 37°C 下，持续 30分钟。
Physical form
0.05 MTris (pH 8.0) 溶液，含 1% 牛血清白蛋白、1 mM MgCl 2 和 15 mM 叠氮化钠
Preparation Note
经过吸附以减少人体样本的背景染色。
Other Notes
用人IgG吸附抗体。
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
Still not finding the right product?
存储类别
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
低风险生物材料
此项目有
商品
ELISpot assay provides qualitative and quantitative information on immune responses, visualizing multiple secretory products from single responding cells.
Rita De Santis et al.
Clinical cancer research : an official journal of the American Association for Cancer Research, 12(7 Pt 1), 2191-2196 (2006-04-13)
ST2146biot is a biotinylated anti-tenascin monoclonal antibody (mAb) to be used for Pretargeted Antibody Guided Radioimmunotherapy (PAGRIT) of solid tumors. In vivo biodistribution studies of (125)I-labeled ST2146biot were done in nude mice transplanted with human HT-29 colon carcinoma and/or human
D J Lewis et al.
European journal of immunology, 21(9), 2087-2094 (1991-09-01)
The immune response to cholera toxin B subunit given orally was studied in 13 human volunteers. A serum IgG and IgA antitoxin response was observed, which was boosted by a second immunization. Using an immunospot assay, cells spontaneously secreting anti-toxin
Development of a Surface Plasmon Resonance?Based
Immunoassay for Listeria monocytogenes
Immunoassay for Listeria monocytogenes
Leonard P, et al.
Journal of Food Protection, 68(4), 728-735 null
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| A1418-1ML | 04061837513848 |
| A1418-.25ML | 04061838352125 |
| A1418-.5ML | 04061837513831 |