General description
藻酸盐裂解酶存在于几种海藻、海洋软体动物以及多种海洋和陆地细菌中。
Application
藻酸盐裂解酶已被用于:
- 酶的预处理
- 去封装细胞
- 消化假单胞菌外多糖,以促进电穿孔
藻酸盐裂解酶可用于分解藻酸盐或海藻酸,也可用于降低粘度。可用于卵泡分离、包封和培养过程中。
Biochem/physiol Actions
藻酸盐裂解酶可积极参与酸性多糖的糖化,以生产生物乙醇。
可将藻酸盐或海藻酸分解成小分子并降低粘度。
Preparation Note
将0.15 ml酶溶液(1 un/ml)加到4.5 ml 0.1%藻酸钠(pH 6.3)中。 37℃孵育30分钟。加入4.65 ml 0.1 N NaOH可终止反应。
Other Notes
在37℃、pH 6.3条件下,一单位酶可使每毫升藻酸钠溶液的A235nm每分钟增加1.0
想查看有关 复合碳水化合物分析酶 的更多信息,请访问 www.sigma-aldrich.com/enzymeexplorer
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
此项目有
W Hashimoto et al.
Journal of bacteriology, 182(16), 4572-4577 (2000-07-27)
A bacterium, Sphingomonas sp. strain A1, can incorporate alginate into cells through a novel ABC (ATP-binding cassette) transporter system specific to the macromolecule. The transported alginate is depolymerized to di- and trisaccharides by three kinds of cytoplasmic alginate lyases (A1-I
Shinji Sakai et al.
Biomaterials, 33(28), 6721-6727 (2012-07-10)
We report a method for wrapping tissues with a pre-established cage-like layer composed of living cells. We encapsulated multicellular aggregates of human hepatoma HepG2 cells as a model of tissues such as pancreatic islets and hepatocyte spheroids in alginate-based hydrogel
Bacteriophages phi MR299-2 and phi NH-4 Can Eliminate Pseudomonas aeruginosa in the Murine Lung and on Cystic Fibrosis Lung Airway Cells
Alemayehu D, et al.
mBio, 3(2) (2012)
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| A1603-100MG | 04061835540525 |
