conjugate
agarose conjugate
Quality Level
antibody product type
primary antibodies
form
suspension
isotype
IgG12b
capacity
≥0.6 mg/mL, gel binding capacity
storage temp.
−20°C
General description
ANTI-FLAG® M1琼脂糖亲和凝胶由纯化的IgG2B单克隆抗体和琼脂糖共价连接而成。
Application
用于纯化N末端FLAG融合蛋白。由于结合过程不依赖Ca2+，蛋白质能采用含EDTA的缓冲液洗脱，也可以使用FLAG多肽或甘油-HLC缓冲液(pH3)通过标准方法洗脱。ANTI-FLAG M1不会和Met-FLAG融合蛋白结合，所以这种树脂不适合纯化未加工处理的细胞质表达融合蛋白。
该亲和凝胶可用于N末端FLAG融合蛋白的钙介导纯化。
免疫沉淀(IP)：适用
洗脱方法：FLAG多肽、甘氨酸、pH3.5 EDTA
更多产品信息请访问我们的FLAG®应用页面。
该亲和凝胶可用于N末端FLAG融合蛋白的钙介导纯化。
免疫沉淀(IP)：适用
洗脱方法：FLAG多肽、甘氨酸、pH3.5 EDTA
更多产品信息请访问我们的FLAG®应用页面。
Biochem/physiol Actions
结合特异性：FLAG 序列
N-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-C自由N末端
N-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-C自由N末端
Features and Benefits
- 通常从粗裂解物中纯化融合蛋白，然后只使用一步层析，纯化成单条带
- 用EDTA温和洗脱，将融合蛋白从亲和树脂上洗脱下来。
- FLAG多肽溶液可以用于FLAG融合蛋白温和地，非变性洗脱。
Physical form
球形琼脂糖悬浮在含10 mM磷酸钠、150mM NaCl (pH7.4)、0.02% (w/v) 叠氮化钠的50%甘油溶液中
Other Notes
ANTI-FLAG® M1并不会和Met-FLAG融合蛋白结合，所以这种树脂不适合纯化未处理的细胞质表达融合蛋白。
Legal Information
ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
低风险生物材料
此项目有
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全球贸易项目编号
| 货号 | GTIN |
|---|---|
| A4596-5ML | 04061833374764 |
| A4596-10ML | 04061833374740 |
| A4596-1ML | 04061838095442 |
| A4596-25ML | 04061833374757 |