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Merck
CN

A6154

抗-兔IgG(全分子)-过氧化物酶 山羊抗

affinity isolated antibody, buffered aqueous solution

别名:

Goat Anti-Rabbit IgG (whole molecule)–HRP

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关于此项目

UNSPSC Code:
12352203
NACRES:
NA.46
MDL number:
Conjugate:
peroxidase conjugate
Clone:
polyclonal
Application:
ELISA (d)
Citations:
489
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biological source

goat

Quality Level

conjugate

peroxidase conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

rabbit

technique(s)

direct ELISA: 1:10,000

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

IgG是糖蛋白抗体,可调控几种免疫应答。靶蛋白的兔IgG通常在多种研究应用中用作一抗。因此,与可检测底物缀合的抗兔IgG二抗是靶蛋白分析的有用工具。山羊抗兔IgG(全分子)-过氧化物酶抗体在缀合前通过与正常血清和兔IgG免疫电泳对兔IgG产生免疫特异性。

Immunogen

纯化的兔IgG

Application

使用HRP标记的山羊抗兔IgG作为二抗,通过蛋白印迹分析C33A细胞裂解物。 对来自结肠粘膜提取物(μg)的蛋白等分试样进行蛋白印迹分析:使用HRP标记的山羊抗兔IgG作为二抗,用TBST以1:5000稀释,室温孵育1小时。
山羊抗兔IgG(全分子)-过氧化物酶抗体用于蛋白印迹(1:6,000)和ELISA检测。
用 HRP 标记的羊抗兔 IgG 作为二抗,对 C33A 细胞裂解物进行免疫共沉淀和免疫印迹分析。

Physical form

0.01 M磷酸盐缓冲液(pH 7.4,含有1%牛血清白蛋白和防腐剂)溶液。

Preparation Note

使用Wilson, M.B.和Nakane, P.K.在Immunofluorescence and Related Staining Techniques, Elsevier/North Holland Biomedical Press, Amsterdam, p215 (1978)中描述的高碘酸盐法制备。

Disclaimer

除非我们的产品目录或产品随附的其他公司文档中另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人或动物。


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Warning

Hazard Classifications

Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

存储类别

12 - Non Combustible Liquids

wgk

WGK 3

法规信息

含少量动物源组分生物产品
低风险生物材料
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全球贸易项目编号

货号GTIN
A6154-.5ML04061835790548
A6154-.25ML04061835805440
A6154-1ML04061837514463
A6154-5X1ML04061837514470