A6255
α-淀粉酶 来源于猪胰腺
PMSF Treated, Type I-A, saline suspension, ≥1000 units/mg protein (E1%/280)
别名:
PPA, al1,4 葡聚糖-4-葡萄糖水解酶，, 猪胎盘β-N-乙酰葡糖胺糖苷酶, 猪胰腺α-淀粉酶
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关于此项目
UNSPSC Code:
12352204
eCl@ss:
32160410
EC Number:
232-565-6
NACRES:
NA.54
MDL number:
Specific activity:
≥1000 units/mg protein (E1%/280)
Biological source:
Porcine pancreas
biological source
Porcine pancreas
Quality Level
type
Type I-A
form
saline suspension
specific activity
≥1000 units/mg protein (E1%/280)
mol wt
51-54 kDa
greener alternative product characteristics
Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
greener alternative category
, Enabling
shipped in
wet ice
storage temp.
2-8°C
General description
分子质量：51-54 kDa。
从猪胰腺中分离的α-淀粉酶是一种糖蛋白。它是由约475个残基组成的一条多肽链（包含2个SH基团和4个二硫键）以及一个紧密结合的Ca2+ （稳定性所必需的）组成的。氯离子是活性和稳定性所必需的。活性pH范围为5.5至8.0，最适pH为7。
从猪胰腺中分离的α-淀粉酶是一种糖蛋白。它是由约475个残基组成的一条多肽链（包含2个SH基团和4个二硫键）以及一个紧密结合的Ca2+ （稳定性所必需的）组成的。氯离子是活性和稳定性所必需的。活性pH范围为5.5至8.0，最适pH为7。
我们致力于为您提供更环保的替代产品，以符合“绿色化学的12项原则”的一项或多项原则要求。当用于淀粉乙醇研究时，该产品经优化可提高能效和防止浪费。有关详细信息，请参阅《Biofiles》的文章。
Application
α-淀粉酶用于水解α-连接的多糖α键，如淀粉和糖原。产品 A6255 来自猪胰腺，为I-A 型。Sigma 公司生产的α-淀粉酶已被用于各种植物研究，如 拟南芥 代谢研究。
Biochem/physiol Actions
α-淀粉酶可水解三个或更多 α-(1,4)连接的 D-葡萄糖单位多糖中的 α-(1,4) 葡聚糖键。天然底物，如淀粉和糖原，被分解成葡萄糖和麦芽糖。来自猪胰腺的α-淀粉酶是一种糖蛋白，由约 475 个残基的单一多肽链组成，含有 2 个 SH 基团和 4 个二硫键以及保持稳定性所必需的紧密结合的 Ca 2 + 。
Physical form
含 3 mM CaCl 的 2.9 M NaCl 悬液
Preparation Note
PMSF处理。2×结晶
Other Notes
有关复合碳水化合物分析 酶的更多信息，请访问 www.sigma-aldrich.com/enzymeexplorer
一个单位将在20℃的pH 6.9下，在3分钟内从淀粉中释放1.0毫克麦芽糖
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signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
法规信息
动植物源性产品
低风险生物材料
此项目有
实验方案
可根据此操作规程测定α-淀粉酶的活性。该α-淀粉酶酶促测定将会指导您完成整个过程及必要的计算。
Follow our procedure for the determination of alpha-Amylase activity. This enzymatic assay of a-Amylase guides you through the entire process and necessary calculations.
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In vitro hydrolysis assays are a key tool in understanding differences in rate and extent of digestion of starchy foods. They offer a greater degree of simplicity and flexibility than dynamic in vitro models or in vivo experiments for quantifiable
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| A6255-100MG | 04061832841380 |
| A6255-10MG | 04061833381939 |
| A6255-25MG | 04061833381946 |