type
Grade VII
Quality Level
form
lyophilized powder
ATPase activity
≥200 units/mg protein
secondary activity
≥50 % of base activity ADPase
composition
Protein, 25-60%
solubility
H2O: soluble 1.0 mg/mL
application(s)
diagnostic assay manufacturing
foreign activity
Acid Phosphatase ≤2% of base activity
storage temp.
−20°C
Application
三磷酸腺苷双磷酸酶用于水解核苷三磷酸和二磷酸。水解有机二磷酸和三磷酸的最佳pH为6,水解无机底物的最佳pH为5.1。Sigma的三磷酸腺苷双磷酸酶已用于血小板聚集的抑制研究。
不同种类的马铃薯(S. tuberosum)中存在至少2种同工酶:一种的ATP酶/ADP酶比例较高(∼10)和另一种较低(∼1)。
反应:ATP → ADP+Pi → AMP+2Pi。
反应:ATP → ADP+Pi → AMP+2Pi。
Biochem/physiol Actions
三磷酸腺苷双磷酸酶存在于所有真核生物和部分原核生物中。 马铃薯三磷酸腺苷双磷酸酶在调节生长和发育中发挥着至关重要的作用。 三磷酸腺苷双磷酸酶在神经刺激后可作为神经递质参与突触ATP的失活,也参与抑制ADP诱导的血小板聚集,防止血栓形成。 其活性需要二价金属离子,在5 mM钙离子存在下观察到最佳活性。
Packaging
按 ATP 酶单位销售。
Physical form
部分纯化的冻干粉,含琥珀酸钾缓冲盐。
Preparation Note
来源于红皮土豆
Other Notes
在pH 6.5、30℃条件下,一单位酶每分钟可从ATP或ADP释放1.0 μmM无机磷酸盐。
该等级具有低 ATP 酶/ADP 酶比率。
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signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
低风险生物材料
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全球贸易项目编号
| 货号 | GTIN |
|---|---|
| A6535-100UN | 04061833382400 |
| A6535-2KU | 04061833382431 |
| A6535-1KU | 04061833382417 |
| A6535-200UN | 04061833382424 |
| A6535-500UN | 04061833382448 |
