form
lyophilized powder
Quality Level
specific activity
≥150 units/mg protein (biuret)
composition
Protein, 5.0-20.0%
UniProt accession no.
foreign activity
adenosine triphosphatase and NADH oxidase ≤0.01%
storage temp.
−20°C
Gene Information
Escherichia coli K12 ... ackA(946775)
General description
乙酸激酶属于乙酸盐和糖激酶/Hsc70/肌动蛋白(ASKHA)结构超家族。它以二聚体形式存在,并在N和C端结构域之间的缝隙中存在有核苷酸结合位点。
Application
在木糖葡糖杆菌的发酵过程以及动力学建模和稳态研究中,来自大肠杆菌的乙酸激酶已被用于评估乙酸激酶的活性。
乙酸激酶可用于将乙酸磷酸化为乙酰磷酸。 来自大肠杆菌的乙酸激酶已被用作ATP再生系统的一部分,用于研究激动剂刺激的转磷脂酰化反应的动力学。
Biochem/physiol Actions
乙酸激酶在糖酵解中起重要作用。乙酸激酶会在ATP和二价阳离子的存在下将乙酸盐磷酸化,并最终导致乙酰辅酶A的生成。乙酸激酶也会参与到丙酸酯、丙酮酸和牛磺酸的代谢。
参与丙酸酯、丙酮酸酯和牛磺酸的代谢。
Physical form
冻干粉,含海藻糖,以及少量磷酸钾、氯化镁和二硫苏糖醇
Other Notes
在pH 7.6、25℃条件下,一单位可在每分钟内将1.0 μmole的乙酸磷酸化成乙酰磷酸。
存储类别
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
Influence of residual ethanol concentration on the growth of Gluconacetobacter xylinus I 2281
Kornmann H, et al.
Applied Microbiology and Biotechnology, 62(2-3), 168-173 (2003)
Urkinase: structure of acetate kinase, a member of the ASKHA superfamily of phosphotransferases
Buss KA, et al.
Journal of Bacteriology, 183(2), 680-686 (2001)
S C Olson et al.
The Journal of biological chemistry, 266(26), 17236-17242 (1991-09-15)
Receptor-linked activation of phospholipase D has been demonstrated recently in a variety of intact cell types including granulocytes, but little is known about the enzyme, its cofactor requirements, and regulation. Using [3H]alkyllysophosphatidylcholine to prelable an endogenous phosphatidylcholine substrate pool in
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| A7437-1KU | 04061832921235 |
| A7437-250UN | 04061832921259 |