A8273
抗 α-淀粉酶 兔抗
fractionated antiserum, lyophilized powder
别名:
Alpha Amylase Antibody, Alpha Amylase Antibody - Anti-α-Amylase antibody produced in rabbit
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关于此项目
Conjugate:
unconjugated
Clone:
polyclonal
Application:
ELISA (i), ODD
Citations:
85
biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
fractionated antiserum
antibody product type
primary antibodies
clone
polyclonal
form
lyophilized powder
species reactivity
human
packaging
vial of 2 mL lyophilized antiserum
technique(s)
Ouchterlony double diffusion: suitable, indirect ELISA: 1:4,000-1:6,000
storage temp.
2-8°C
target post-translational modification
unmodified
Gene Information
human ... AMY1A(276)
General description
α-淀粉酶主要在唾液腺和胰腺中大量产生。在空肠和乳腺中也可表达。编码α淀粉酶的基因是位于1号染色体短臂上的AMY1和AMY2。AMY1负责在唾液和乳腺中产生这种酶，而AMY2则在胰腺中合成这种酶。AMY1也在某些肿瘤组织中表达。哺乳动物淀粉酶由三个结构域（A、B和C）组成。结构域A包含具有两个天冬氨酸和一个谷氨酸残基的活性位点。结构域B毗邻活性位点区，它是维持蛋白质构象是必不可少的结构。结构域C涉及催化机理。
Immunogen
人唾液α-淀粉酶。
Application
兔源抗α-淀粉酶抗体可用于免疫荧光和免疫组织化学分析。
兔源抗α淀粉酶抗体还被用于免疫细胞化学分析。
将小鼠胰腺切片固定在4％多聚甲醛中，并使用兔抗淀粉酶抗体以1：100的稀释度用于免疫组织化学。
Biochem/physiol Actions
α--淀粉酶对于催化淀粉消化的第一步是必不可少的，淀粉消化是人类饮食中碳水化合物的主要来源。α-淀粉酶水解多糖，最终生成的主要产物为麦芽糖、麦芽三糖和极限糊精。
该抗血清对在人唾液和人胰腺提取物中发现的人α-淀粉酶具有特异性。未观察到与其他人唾液蛋白或胰腺提取蛋白的反应。
Physical form
由 0.01M 的磷酸缓冲盐溶液 (pH 7.2) 冻干
Preparation Note
用 2 mL 去离子水复溶。
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存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
低风险生物材料
此项目有
Joris van Arensbergen et al.
PloS one, 12(2), e0171508-e0171508 (2017-02-23)
Lineage-selective expression of developmental genes is dependent on the interplay between activating and repressive mechanisms. Gene activation is dependent on cell-specific transcription factors that recognize transcriptional enhancer sequences. Gene repression often depends on the recruitment of Polycomb group (PcG) proteins
Maho Kodama et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 56(1), 33-44 (2007-09-19)
In this study, we describe pancreatic cell ontogeny in renal capsule-transplanted embryonic stem cells (ES) after injury by streptozocin (STZ), showing pancreatogenesis in situ. Seven-week-old female BALB/c nude mice were treated with either a single 175- or 200-mg/kg STZ dose
Ultrastructural analysis of pancreatic acinar cells from mice fed on genetically modified soybean
Malatesta M, et al.
Journal of Anatomy, 201(5), 409-415 (2002)
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| A8273-1VL | 04061833391372 |