biological source
goat
Quality Level
conjugate
peroxidase conjugate
antibody form
IgG fraction of antiserum
antibody product type
secondary antibodies
clone
polyclonal
form
buffered aqueous solution
technique(s)
direct ELISA: 1:10,000
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
General description
IgG是一种单体,是一种在血液中循环的抗体,可以协助吞噬破坏外来微生物。它在抗体依赖性细胞介导的细胞毒性中发挥重要作用,并且还与II型和III型超敏反应有关。抗兔IgG(全分子)过氧化物酶偶联抗体可用于pAb-ELISA。兔抗IgG抗体与过氧化物酶(在TBS、0.5% BSA中以1:2500稀释)可用于蛋白质印迹。该抗体还用作间接ELISA法进行蛤蚌毒素分析的检测器。山羊抗兔IgG过氧化物酶缀合物与所有兔免疫球蛋白发生特异性反应。
Immunogen
纯化兔 IgG。
Application
抗兔 IgG(全分子)过氧化物酶结合物抗体用于蛋白印迹法、间接 ELISA、免疫印迹和免疫检测。
用 HRP 标记的羊抗兔 IgG 作为二抗,对 C33A 细胞裂解物进行免疫共沉淀和免疫印迹分析。
用HRP标记的山羊抗兔IgG作为二抗,对小鼠肠、肝和脾的冰冻切片 (10um) 进行免疫组化分析。在与二抗孵育之前,用MeOH/过氧化氢30%的混合物处理切片,以阻断内源过氧化物酶。
Physical form
0.01 M磷酸盐缓冲液(pH 7.4,含有1%牛血清白蛋白和防腐剂)的溶液。
Preparation Note
按照Wilson, M.B.,和Nakane, P.K.在由阿姆斯特丹Elsevier/北荷兰生物医学出版社出版的免疫荧光及相关染色技术(1978)一书中所描述的高碘酸盐法而制备。
Disclaimer
除非我们的目录或随附于产品的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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Warning
hcodes
Hazard Classifications
Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1
存储类别
12 - Non Combustible Liquids
wgk
WGK 3
法规信息
含少量动物源组分生物产品
常规特殊物品
低风险生物材料
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全球贸易项目编号
| 货号 | GTIN |
|---|---|
| A8275-.5ML | 04061835805716 |
| A8275-2ML | 04061835805723 |
| A8275-1ML | 04061833391389 |

