A9357
Anti-alpha-Actin Antibody, Cardiac Muscle (ACTC1) Antibody
mouse monoclonal, AC1-20.4.2
别名:
Anti-ACTC, Anti-ACTC1, Anti-Actin alpha cardiac muscle 1, Anti-CMD1R, Anti-CMH11
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关于此项目
UNSPSC Code:
12352203
NACRES:
NA.41
Conjugate:
unconjugated
Clone:
AC1-20.4.2, monoclonal
Application:
ELISA (i), ICC, IHC (p), WB
Citations:
19
产品名称
Anti-Actin, Cardiac antibody, Mouse monoclonal, clone AC1-20.4.2, purified from hybridoma cell culture
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
AC1-20.4.2, monoclonal
form
buffered aqueous solution
mol wt
antigen ~42 kDa
species reactivity
rat, human, bovine, chicken, mouse
concentration
~1.0 mg/mL
technique(s)
immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 10-20 μg/mL using digested rat heart tissue, indirect ELISA: suitable, western blot: suitable
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... ACTC1(70)
mouse ... Actc1(11464)
rat ... Actc1(29275)
General description
Actins are cytoskeletal proteins that are widely expressed in eukaryotes. These proteins primarily regulate various cellular functions such as muscle contraction, mRNA synthesis and apoptosis among many others. ACTC1 is the predominant actin isoform form in adult cardiac tissues and alterations in this isoform have been associated with hypertrophic cardiomyopathy and congenital heart disease . Monoclonal Anti-Actin, Cardiac antibody is specific for human, bovine, chicken, rat, and mouse cardiac α actin.
Immunogen
synthetic NH2-terminal decapeptide of cardiac isoform of actin.
Application
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)
Western Blotting (1 paper)
Immunohistochemistry (1 paper)
Western Blotting (1 paper)
Monoclonal Anti-Actin, Cardiac antibody is suitable for use in immunocytochemistry, immunoblot (approx. 42 kDa), indirect ELISA, and western blot. The antibody may also be used in immunohistochemistry (using formalin-fixed, paraffin-embedded sections, or using a conc. of 10-20 μg/mL in digested rat heart tissue).
Protein samples isolated from cardiac sections or cardiac myocytes were subjected to western blot using monoclonal mouse anti-actin antibody as the primary at a dilution of 1:2000.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
wgk
WGK 1
存储类别
12 - Non Combustible Liquids
法规信息
常规特殊物品
低风险生物材料
常规特殊物品
此项目有
Iel-Yong Sung et al.
International journal of medical sciences, 13(11), 841-852 (2016-11-24)
The purpose of the present study was to investigate the in vitro cardiomyogenic differentiation potential of human dental follicle-derived stem cells (DFCs) under the influence of suberoylanilide hydroxamic acid (SAHA), a member of the histone deacetylase inhibitor family, and analyze
Mirco Müller et al.
Cellular and molecular life sciences : CMLS, 69(20), 3457-3479 (2012-05-31)
Inherited cardiomyopathies are caused by point mutations in sarcomeric gene products, including α-cardiac muscle actin (ACTC1). We examined the biochemical and cell biological properties of the α-cardiac actin mutations Y166C and M305L identified in hypertrophic cardiomyopathy (HCM). Untagged wild-type (WT)
W W Franke et al.
Differentiation; research in biological diversity, 60(4), 245-250 (1996-07-01)
We describe three murine monoclonal antibodies (mAbs) raised against a synthetic decapeptide representing the aminoterminal sequence of the cardiac/ fetal isoform of sarcomeric alpha-actin. When used for immunoblotting or histological immunolocalization, these mAbs distinguish cardiac/fetal alpha-actin from skeletal muscle alpha-actin
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| A9357-100UL | 04061837718984 |
| A9357-200UL | 04061833402382 |