产品名称
BME培养基, With Earle′s salts and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture
Quality Level
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
components
L-glutamine: no
NaHCO3: yes
Earle’s salts (5% CO2): yes
shipped in
ambient
storage temp.
2-8°C
General description
Eagle基础培养基（BME）是应用最广泛的合成细胞培养基之一。组织培养协会建议，术语“Eagle基础培养基”仅用于指代专为支持海拉细胞而开发的配方。BME先前已用于单层培养研究，以评估正常（WI-38）和转化（小鼠和海拉）细胞的生长反应。BME是杜氏改良Eagle培养基（DME）和Eagle最低必需培养基（MEM）的前体。
Application
Eagle培养基已用于：
- 作为耳蜗器官型培养物和水杨酸治疗的培养基组分
- 维持小脑颗粒神经元（CGN），以确定是否存在调节轴突线粒体分布和/或群体动态的系统
- 制备用于切片叠加分析的皮层切片、无血清培养基和切片培养基，以研究细胞外信号对神经元发育的影响
Preparation Note
添加 0.292g/L L-谷氨酰胺。
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
商品
钙是一种离子稳定的二价阳离子，在细胞培养基中具有有益和有毒的双重属性，可作为一系列细胞培养基的组成部分。
用于传代和分装悬浮细胞系的细胞培养方案
Calcium is an ionically stable divalent cation with important beneficial and toxic properties in cell culture. It is a component of a wide range of cell culture media.
Franck Polleux et al.
Science's STKE : signal transduction knowledge environment, 2002(136), pl9-pl9 (2002-06-13)
We have developed a technique that allows characterization and identification of extracellular signals that regulate various aspects of neuronal differentiation. In this in vitro assay, dissociated cells isolated from the developing cerebral wall are labeled and cultured over organotypic cortical
G M Seigel et al.
Molecular and cellular neurosciences, 85, 202-210 (2017-11-08)
Microglia are glial-immune cells that are essential for the function and survival of the central nervous system. Microglia not only protect neural tissues from immunological insults, but also play a critical role in neural development and repair. However, little is
Bethtrice Thompson et al.
PloS one, 11(2), e0148619-e0148619 (2016-02-20)
Bone continuously undergoes remodeling by a tightly regulated process that involves osteoblast differentiation from Mesenchymal Stem Cells (MSC). However, commitment of MSC to osteoblastic lineage is a poorly understood process. Chromatin organization functions as a molecular gatekeeper of DNA functions.
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| B1522-500ML | 04061833426159 |
| B1522-6X500ML | 04061833426166 |