产品名称
SIGMAFAST™ BCIP®/NBT, tablet
form
tablet
Quality Level
solubility
water: 10 mL, clear, yellow
storage temp.
−20°C
Application
SIGMAFAST BCIP®/NBT已被用作以下底物：
- 用于免疫印迹的抗小鼠IgG偶联碱性磷酸酶
- 用于原代成骨细胞组织化学染色中碱性磷酸酶
- 骨髓基质细胞碱性磷酸酶染色
Preparation Note
将一片溶于10 ml去离子水中，得到含有BCIP/NBT的即用型缓冲溶液，pH 9.5。
Legal Information
BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany
SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC
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存储类别
11 - Combustible Solids
实验方案
本实验方案通过对石蜡包埋的组织切片中的特定抗原进行染色和成像，完成整个免疫组织化学（IHC）操作步骤。
5-溴-4-氯-3-吲哚基磷酸盐（BCIP）/ 硝基蓝四唑（NBT）是一种理想的不溶性底物，可与碱性磷酸酶一起使用。该系统产生蓝紫色产物。可以目视观察到强烈的颜色，非常稳定，并且在暴露于光时不褪色。
TrueGel3D® Hydrogel Plate protocol guides high-throughput culture of human adipose MSCs for screening applications.
商品
硝基蓝四唑（NBT）可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐（BCIP）一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物，其颜色为蓝至紫色，可以通过视觉观察到。
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
Andriy Didovyk et al.
ACS synthetic biology, 6(12), 2198-2208 (2017-08-11)
Cell-free gene expression systems are emerging as an important platform for a diverse range of synthetic biology and biotechnology applications, including production of robust field-ready biosensors. Here, we combine programmed cellular autolysis with a freeze-thaw or freeze-dry cycle to create
Sialic acid on herpes simplex virus type 1 envelope glycoproteins is required for efficient infection of cells
Teuton JR and Brandt CR
Journal of Virology, 81(8), 3731-3739 (2007)
Mitogen-activated protein kinase phosphatase 1 regulates bone mass, osteoblast gene expression, and responsiveness to parathyroid hormone
Mahalingam CD, et al.
The Journal of Endocrinology, 211(2), 145-156 (2011)
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| B5655-5TAB | 04061835553952 |
| B5655-25TAB | 04061835553945 |