B6024
5-溴-4-氯-3-吲哚基-β-吡咯-D-半乳糖苷
tablet, suitable for identification of lac+ bacterial colonies
别名:
5-溴-4-氯-3-吲哚基-β-吡咯-D-半乳糖苷, BCIG, X-Gal
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关于此项目
经验公式（希尔记法）:
C14H15BrClNO6
化学文摘社编号:
分子量:
408.63
NACRES:
NA.83
PubChem Substance ID:
UNSPSC Code:
12352204
EC Number:
230-640-8
MDL number:
Beilstein/REAXYS Number:
1402009
产品名称
5-溴-4-氯-3-吲哚基-β-吡咯-D-半乳糖苷, tablet
form
tablet
suitability
suitable for identification of lac+ bacterial colonies
storage temp.
−20°C
SMILES string
OC[C@H]1O[C@@H](Oc2c[nH]c3ccc(Br)c(Cl)c23)[C@H](O)[C@@H](O)[C@H]1O
InChI
1S/C14H15BrClNO6/c15-5-1-2-6-9(10(5)16)7(3-17-6)22-14-13(21)12(20)11(19)8(4-18)23-14/h1-3,8,11-14,17-21H,4H2/t8-,11+,12+,13-,14-/m1/s1
InChI key
OPIFSICVWOWJMJ-AEOCFKNESA-N
General description
5-溴-4-氯-3-吲哚基 β-D-吡喃半乳糖苷，通常称为 X-Gal，是 β-半乳糖苷酶的组织化学底物。
Application
5-溴-4-氯-3-吲哚基 β-D-吡喃半乳糖苷已用于制备 X-Gal 储备液，并将其添加到 LB 琼脂平板中，用于蓝白色筛选转化子。
X-Gal是β-半乳糖苷酶的发色底物，可产生丰富的蓝色，与背景相比可以直观地检测到。 X-Gal是lac+基因型重组细菌菌落蓝白斑筛选的首选基质。
Biochem/physiol Actions
X-gal 通常用于转基因活性的原位检测。
Preparation Note
每片含 5 mg 底物
通过将 X-Gal 溶解在二甲基甲酰胺中制备储备液（100 mg/ml），应将其储存在 −20°C 并避光。无需灭菌。
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存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
涉药品监管产品
此项目有
Analysis of Transgene Expression
Transactions of the American Clinical and Climatological Association, 543-564 (2014)
Creation of recombinant antigen-binding molecules derived from hybridomas secreting specific antibodies
Fields C, et al.
Nature Protocols, 8(6), 1125-1125 (2013)
Khanh-Van Tran et al.
Cell metabolism, 15(2), 222-229 (2012-02-14)
Adipose tissue expansion involves the enlargement of existing adipocytes, the formation of new cells from committed preadipocytes, and the coordinated development of the tissue vascular network. Here we find that murine endothelial cells (ECs) of classic white and brown fat
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| B6024-50TAB | 04061833627839 |
| B6024-10TAB | 04061833291405 |