B6777
5-溴-4-氯-3-吲哚磷酸 对甲苯胺盐
BioReagent, ≥99%, powder, suitable for molecular biology
别名:
BCIP® 对甲苯胺盐, X-磷酸盐 对甲苯胺盐
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关于此项目
经验公式（希尔记法）:
C8H6BrClNO4P · C7H9N
化学文摘社编号:
分子量:
433.62
NACRES:
NA.83
PubChem Substance ID:
UNSPSC Code:
12352204
EC Number:
229-506-1
MDL number:
产品名称
5-溴-4-氯-3-吲哚磷酸 对甲苯胺盐, BioReagent, Molecular Biology, powder, ≥99%
grade
Molecular Biology
Quality Level
product line
BioReagent
assay
≥99%
form
powder
solubility
DMF: 20 mg/mL, H2O: insoluble
foreign activity
Protease, none detected
storage temp.
−20°C
SMILES string
Cc1ccc(N)cc1.OP(O)(=O)Oc2c[nH]c3ccc(Br)c(Cl)c23
InChI
1S/C8H6BrClNO4P.C7H9N/c9-4-1-2-5-7(8(4)10)6(3-11-5)15-16(12,13)14;1-6-2-4-7(8)5-3-6/h1-3,11H,(H2,12,13,14);2-5H,8H2,1H3
InChI key
QEIFSLUFHRCVQL-UHFFFAOYSA-N
General description
碱性磷酸酶的组织化学底物。
Application
用于碱性磷酸酶标记分子的比色检测。
用于碱性磷酸酶标记分子的比色检测，可使用5-溴-4-氯-3-吲哚基磷酸酯（BCIP）和硝基蓝四唑（NBT）。BCIP/NBT底物系统具有多种功能并用于多种应用，包括Northern Southern和Western blotting、原位杂交和免疫组化。
BCIP以两种盐形式提供：可溶于水的二钠盐和可溶于二甲基甲酰胺的对甲苯胺形式。这些盐形式可用于制备与NBT及反应缓冲液组合的储存溶液，并形成碱性磷酸酶的底物溶液。当与碱性磷酸酶一起孵育时，该底物系统可产生不溶的NBT二甲嗪产物，具有容易被观察到的紫色。为了方便起见，BCIP和NBT的混合物是以一种易溶解的片剂形式或作为即用型液体而提供的。
BCIP以两种盐形式提供：可溶于水的二钠盐和可溶于二甲基甲酰胺的对甲苯胺形式。这些盐形式可用于制备与NBT及反应缓冲液组合的储存溶液，并形成碱性磷酸酶的底物溶液。当与碱性磷酸酶一起孵育时，该底物系统可产生不溶的NBT二甲嗪产物，具有容易被观察到的紫色。为了方便起见，BCIP和NBT的混合物是以一种易溶解的片剂形式或作为即用型液体而提供的。
Packaging
Bottomless glass bottle. Contents are inside inserted fused cone.
Legal Information
BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany
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11 - Combustible Solids
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全球贸易项目编号
| 货号 | GTIN |
|---|---|
| B6777-100MG | 04061833435847 |
| B6777-500MG | 04061833435861 |