C1764
胶原酶 来源于溶组织梭菌
0.2 μm filtered, suitable for release of physiologically active rat epididymal adipocytes, Type II-S, 0.5-5.0 FALGPA units/mg solid, ≥125 CDU/mg solid
别名:
梭菌肽酶 A
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关于此项目
化学文摘社编号:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-582-9
MDL number:
Specific activity:
≥125 CDU/mg solid, 0.5-5.0 FALGPA units/mg solid
Quality Level
sterility
0.2 μm filtered
type
Type II-S
form
lyophilized powder
specific activity
≥125 CDU/mg solid, 0.5-5.0 FALGPA units/mg solid
mol wt
68-125 kDa
suitability
suitable for release of physiologically active rat epididymal adipocytes
application(s)
diagnostic assay manufacturing
storage temp.
−20°C
Application
胶原酶已用于动脉组织的制备，用于晚期糖基化终产物 (AGE) 的研究。这种酶还与其他蛋白酶一起用于人类肿瘤、小鼠肾、人脑、肺上皮和许多其他组织的解聚。在肝脏和肾脏灌注研究、胰腺的消化和非实质肝细胞的分离中也有效。也适用于大鼠附睾脂肪垫脂肪细胞的制备。
Biochem/physiol Actions
有效地从组织中释放细胞需要胶原酶和中性蛋白酶的共同作用。每摩尔酶用 4 克原子钙 (Ca2+) 激活胶原酶。一般认为培养滤液至少含有 7 种不同的蛋白酶，分子量范围为 68-130 kDa（最适用 pH：6.3-8.8）。该酶识别序列-R-Pro-8-X-Gly-Pro-R-，其中 X 是最常见的中性氨基酸。
每摩尔胶原蛋白酶被4g原子钙活化。 它被乙二醇-双（β-氨基乙基醚）-N，NN′,N′-四乙酸，β-巯基乙醇、谷胱甘肽、巯基乙酸和8-羟基喹啉抑制。
Preparation Note
本品由溶组织梭菌的 Ⅱ 型 (C6885) 胶原酶制备而成。通常在 37°C 下用 TESCA 缓冲液（含 50 mM TES，0.36 mM 氯化钙，pH 7.4）制备 1-2 mg/mL 溶液。溶液还含有梭菌蛋白酶、非特异性中性蛋白酶和胰蛋白酶活性。
Other Notes
一个胶原蛋白消化单元（CDU）从牛跟腱胶原蛋白中释放的肽链量和1.0 μ亮氨酸，5小时，pH7.4,37℃，在钙离子存在下的茚三酮颜色相同。一个 FALGPA水解单元每分钟，25℃，水解1.0 μmole 呋喃基丙烯酰-Leu-Gly-Pro-Ala。一个中性蛋白酶单元水解酪蛋白，产生颜色和1.0 μmole 酪氨酸每5小时，pH7.5,37℃下产生的相同。一个梭菌蛋白酶单元在DTT存在下，pH7.6,25℃，每分钟水解1.0 μmole BAEE。
Disclaimer
供应的产品可在-20°C 下可稳定保存一年。在 37°C、50°C 和-20°C 条件下储存 30 天，FALGPA 或蛋白酶活性无损失。快速冷冻 (10 mg/mL) 并在-20°C 下冷冻时，粗胶原酶溶液是稳定的。进一步冻融循环将破坏溶液。置于冰上 7 小时后，产品仍保持 100% 活性。
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signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
法规信息
常规特殊物品
此项目有
实验方案
在测定胶原酶活性时，使用N-（3-[2-呋喃基]丙烯酰基）-Leu-Gly-Pro-Ala在345nm处进行连续分光光度法测定。胶原酶水解胶原肽键。
To measure collagenase activity, N-(3-[2-Furyl]acryloyl)-Leu-Gly-Pro-Ala is used in a continuous spectrophotometric rate determination at 345 nm. Collagenase hydrolyzes collagen peptide bonds.
Jessica M Kemppainen et al.
Biomaterials, 31(2), 279-287 (2009-10-13)
It has been widely postulated that scaffold permeability has a significant influence on chondrogenesis. However, since permeability has not been rigorously controlled in previous studies, there is no definitive conclusion as to how permeability affects cartilage regeneration by primary chondrocytes
Sachin S Thakur et al.
Experimental eye research, 194, 108006-108006 (2020-03-21)
Vitreous liquefactive processes play an integral role in ocular health. Knowledge of the degree of liquefaction would allow better monitoring of ocular disease progression and enable more informed therapeutic dosing for an individual patient. Presently this process cannot be monitored
Jeffrey Dock et al.
PloS one, 12(8), e0182498-e0182498 (2017-08-24)
A hallmark of human immunosenescence is the accumulation of late-differentiated memory CD8+ T cells with features of replicative senescence, such as inability to proliferate, absence of CD28 expression, shortened telomeres, loss of telomerase activity, enhanced activation, and increased secretion of
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| C1764-50MG | 04061835555819 |