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Merck
CN

C4129

α-糜蛋白酶 来源于牛胰腺

Type II, lyophilized powder, ≥40 units/mg protein

别名:

α-chymotrypsin A and B, alpha-chymotrypsin

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关于此项目

化学文摘社编号:
UNSPSC Code:
12352204
eCl@ss:
42010112
EC Number:
232-671-2
NACRES:
NA.54
MDL number:
Specific activity:
≥40 units/mg protein
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type

Type II

Quality Level

form

lyophilized powder

specific activity

≥40 units/mg protein

mol wt

25 kDa

composition

protein, ≥85%

color

white to off-white

UniProt accession no.

application(s)

diagnostic assay manufacturing

storage temp.

−20°C

Gene Information

cow ... CTRB1(618826)

Application

sigma 公司的酶已被用于研究糖基化 α-胰凝乳蛋白酶的结构 - 功能关系,使用固定化金属离子亲和层析(IMAC)和固定化金属离子亲和毛细管电泳(IMACE)。

Biochem/physiol Actions

α-胰凝乳蛋白酶是丝氨酸肽酶,并且具有通过二硫键连接的三条多肽链(A 链-13 残基,B 链-131 残基和 C 链-97 残基)中包含的 241 个氨基酸残基。发现该酶的分子量为 25kDa。pI 是 8.75。它选择性地水解酪氨酸、苯丙氨酸、色氨酸和亮氨酸的 C-末端侧的肽键。Ca2+ 激活和稳定酶。该酶被二异丙基氟磷酸盐(DFP)、甲苯磺酰氟(PMSF)、N-对甲苯磺酰基-L-苯丙氨酸氯甲基酮(TPCK)、胰凝乳蛋白酶抑制剂、抑肽酶、α1-抗胰蛋白酶和 α2-巨球蛋白、10mM Cu2+ 和 Hg2+ 抑制。
一种丝氨酸蛋白酶,能将与芳香烃或其他大型疏水性侧链(Tyr、Trp、Phe、Met、Leu)结合的肽从键的羧基端水解。

Preparation Note

以 3×结晶的胰凝乳蛋白酶原为原料生产

Analysis Note

通过 E1%/280 确定的蛋白质

Other Notes

一个单元将在 pH 7.8,25℃下,每分钟水解 1.0 μmole BTEE。


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signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

存储类别

11 - Combustible Solids

wgk

WGK 1

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

法规信息

动植物源性产品
低风险生物材料

此项目有



历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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实验方案

按照我们的程序进行胰凝乳蛋白酶活性测定。该α-胰凝乳蛋白酶测定将会指导您完成整个过程以及必要的计算。

Follow our procedure for the determination of a chymotrypsin activity. This enzymatic assay of alpha chymotrypsin guides you through the entire process and necessary calculations.

商品

Analytical Enzyme Chymotrypsin: Chymotrypsin is produced in the acinar cells of the pancreas as the inactive precursor, chymotrypsinogen.


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全球贸易项目编号

货号GTIN
C4129-10G04061833491676
C4129-500MG04061833491706
C4129-1G04061833491683
C4129-250MG04061833491690