C4879
α-糜蛋白酶原A 来源于牛胰腺
essentially salt-free, lyophilized powder
别名:
chymotrypsin A zymogen
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关于此项目
Specific activity:
≥40 units/mg solid
Biological source:
bovine pancreas
biological source
bovine pancreas
Quality Level
type
Type II
form
essentially salt-free, lyophilized powder
specific activity
≥40 units/mg solid
mol wt
25,656 Da by calculation
purified by
6× crystallization
solubility
1 mM HCl: soluble 10 mg/mL, clear, colorless
UniProt accession no.
foreign activity
α-chymotrypsin ≤1 U/mg (prior to activation by trypsin)
storage temp.
−20°C
Gene Information
cow ... CTRB1(618826)
General description
来自牛胰腺的胰凝乳蛋白酶原是一种含有5个二硫键的酶原。它的等电点是8.97。
Application
α-来自牛胰腺的胰凝乳蛋白酶原A已作为蛋白质模型用于结晶重现性研究。它还被用于质谱研究前α-麦醇溶蛋白的水解。
Sigma公司的这种酶已用于使用近红外（NIR）光谱技术无创测定固态蛋白质构象的过程中。它被用作研究蛋白质在聚合物/聚合物双水相体系中的分配。这种酶也被用作自相互作用色谱应用，以测试蛋白质渗透第二维利系数的快速测量。在该技术中，将蛋白质固定在色谱微粒上，并使用等度替代测定其保留时间。
Biochem/physiol Actions
胰凝乳蛋白酶原A需要有限的蛋白水解才能激活。胰凝乳蛋白酶原A可由胰蛋白酶和胰凝乳蛋白酶激活（自溶激活）以形成 m α、β、γ、δ和π胰凝乳蛋白酶（取决于激活条件）。胰凝乳蛋白酶原是一种蛋白酶，优先裂解芳香族氨基酸（包括色氨酸、酪氨酸和苯丙氨酸）羧基侧的肽。它也水解亮氨酸、蛋氨酸和丙氨酸羧基侧的肽。
一种丝氨酸蛋白酶，水解肽键与肽键羧基末端的芳香族或大型疏水侧链 (Tyr、Trp、Phe、Met) 的肽键。
Other Notes
有关胰凝乳蛋白酶的更多信息，请访问 www.sigma-aldrich.com/enzymeexplorer
活化为硝酸凝乳蛋白酶后，在 pH 7.8、25°C 条件下，1 个单位将每分钟水解 1.0 μmol BTEE。
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
动植物源性产品
低风险生物材料
此项目有
商品
Analytical Enzyme Chymotrypsin: Chymotrypsin is produced in the acinar cells of the pancreas as the inactive precursor, chymotrypsinogen.
Curtiss P Schneider et al.
The journal of physical chemistry. B, 113(7), 2050-2058 (2009-02-10)
The relatively new technique of vapor pressure osmometry was utilized to determine the preferential interaction of five common solution additives (arginine HCl, guanidine HCl, glycerol, glucose, and urea) using three different model proteins (BSA, lysozyme, and alpha-chymotrypsinogen). Results for guanidine
Curtiss P Schneider et al.
The journal of physical chemistry. B, 115(22), 7447-7458 (2011-05-17)
L-Arginine hydrochloride is a very important aggregation suppressor for which there has been much attention given regarding elucidating its mechanism of action. Little consideration, however, has been given toward other salt forms besides chloride, even though the counterion likely imparts
Peter M Tessier et al.
Biophysical journal, 82(3), 1620-1631 (2002-02-28)
Weak protein interactions are often characterized in terms of the osmotic second virial coefficient (B(22)), which has been shown to correlate with protein phase behavior, such as crystallization. Traditional methods for measuring B(22), such as static light scattering, are too
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| C4879-100MG | 04061833493229 |
| C4879-1G | 04061833493298 |
| C4879-250MG | 04061832700076 |
| C4879-5G | 04061833493359 |