form
lyophilized powder
Quality Level
specific activity
~40,000 units/mg protein
mol wt
PEG 5,000
composition
Protein, ~50% E405
extent of labeling
~40 mol PEG per mol protein
matrix attachment
secondary amine linkage.
storage temp.
−20°C
Application
经 PEG-过氧化氢酶处理的肺动脉内皮细胞可有效抑制 20-HETE（羟基二十碳四烯酸）诱导的荧光增强。但实验排除了 DCF（二氯荧光素）潜在的非特异性荧光。本实验研究了 20-HETE 对超氧化物产生和 NADPH 氧化酶活化的影响。
Biochem/physiol Actions
来自牛肝的过氧化氢酶催化 H 2 O 2 分解为水和氧。是一种四聚体，由分子量为 60 kDa 的四个相等的亚单位组成。每个亚基均含有与血红素 IX 基团结合的铁。该酶还能与靠近血红素基团的 NADP 强烈结合。过氧化氢酶活性在 pH 4.0-8.5 范围内恒定。pI 为 5.4。3-氨基-1-H-1,2,4 三唑、氰化物、叠氮化物、羟胺、溴化氰、2-巯基乙醇、二硫苏糖醇、联茴香胺和硝酸盐可抑制酶活性。用抗坏血酸或抗坏血酸/Cu 2 + 孵育过氧化氢酶会导致过氧化氢酶分子的降解。它不需要任何激活剂。
Packaging
基于蛋白质含量的包装规格
Physical form
含 PEG 加 5% 柠檬酸盐缓冲盐
Other Notes
来自牛肝脏的过氧化氢酶偶联甲氧基-聚乙二醇。
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
动植物源性产品
低风险生物材料
此项目有
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| 货号 | GTIN |
|---|---|
| C4963-2MG | 04061833494158 |