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C5838

单克隆抗肌动蛋白抗体

clone AC-40, purified immunoglobulin, buffered aqueous solution

别名:

Monoclonal Anti-Actin

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关于此项目

NACRES:
NA.41
UNSPSC Code:
12352203
Conjugate:
CY3 conjugate
Clone:
AC-40, monoclonal
Application:
IF (d)
Citations:
9
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biological source

mouse

Quality Level

200

conjugate

CY3 conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

AC-40, monoclonal

form

buffered aqueous solution

mol wt

antigen 42 kDa

species reactivity

human, Xenopus, hamster, mouse, guinea pig, rat, rabbit, snail, sheep, canine, carp, chicken, pig, bovine, viper, goat

technique(s)

direct immunofluorescence: 1:75 using cultured human or chicken fibroblasts

isotype

IgG2a

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... ACTA1(58), ACTA1(58), ACTA1(58), ACTA2(59), ACTA2(59), ACTA2(59), ACTB(60), ACTC1(70), ACTC1(70), ACTC1(70), ACTG1(71), ACTG1(71), ACTG1(71), ACTG2(72), ACTG2(72), ACTG2(72)
mouse ... Acta1(11459), Acta1(11459), Acta1(11459), Acta2(11475), Acta2(11475), Acta2(11475), Actb(11461), Actb(11461), Actb(11461), Actc1(11464), Actc1(11464), Actc1(11464), Actg1(11465), Actg1(11465), Actg1(11465), Actg2(11468), Actg2(11468), Actg2(11468)
rat ... Acta1(29437), Acta1(29437), Acta1(29437), Acta2(81633), Acta2(81633), Acta2(81633), Actb(81822), Actb(81822), Actb(81822), Actc1(29275), Actc1(29275), Actc1(29275), Actg1(287876), Actg1(287876), Actg1(287876), Actg2(25365), Actg2(25365), Actg2(25365)

General description

该抗体可识别在所有肌动蛋白亚型中均保守的C末端表位。它能够特异性标记各种组织和物种中的肌动蛋白。 被抗体识别的表位能够耐受甲醛固定和石蜡包埋。锌-福尔马林、B5、乙醇、methacarn、Brunnel's或Bouin's溶液也可用作固定液。
Monoclonal Anti-Actin (mouse IgG2a isotype) is derived from the AC-40 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Actin has six isoforms. Four of them represent the differentiation markers of muscle tissues and two are found practically in all cells. There are three α-actins (α-skeletal, α-cardiac and α-smooth muscle), one β-actin (β-nonmuscle) and two γ-actins (γ-smooth muscle and γ-nonmuscle).

Immunogen

与多抗原肽（MAP）骨架连接的合成肌动蛋白C末端肽

Application

已使用肌动蛋白单克隆抗体作为一抗，对MDCK细胞裂解物进行蛋白质免疫印迹分析。
肌动蛋白单克隆抗体被用于Cos-7细胞裂解物的蛋白质免疫印迹分析，以确保等量蛋白质上样。
Monoclonal Anti-Actin-Cy3 antibody has been used in immunofluorescent staining.

Biochem/physiol Actions

Actin and myosin are constituents of many cell types and are involved in a myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis and cytokinesis.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Other Notes

To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.

Legal Information

Distributed under license from Amersham Biosciences Limited.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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存储类别

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

常规特殊物品
低风险生物材料

此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
067M4858V
092M4799
118K4832
038K4835
095K4840

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全球贸易项目编号

货号GTIN
C5838-.2ML04061837719936