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Merck
CN

C6198

抗-肌动蛋白, α-平滑肌- Cy3抗体,小鼠单克隆

clone 1A4, purified from hybridoma cell culture

别名:

单克隆抗-肌动蛋白,α-平滑肌, α-平滑肌肌动蛋白- Cy3抗体, α-肌动蛋白2 Cy3, SMA

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关于此项目

NACRES:
NA.43
UNSPSC Code:
12352203
Conjugate:
CY3 conjugate
Clone:
1A4, monoclonal
Application:
IHC (p)
Citations:
544
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biological source

mouse

Quality Level

conjugate

CY3 conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

1A4, monoclonal

form

buffered aqueous solution

mol wt

antigen ~42 kDa

species reactivity

human, mouse, rat, chicken, frog, canine, rabbit, guinea pig, goat, bovine, sheep, snake

storage condition

protect from light

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200 using human tonsil or appendix

isotype

IgG2a

UniProt accession no.

application(s)

research pathology

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... ACTA2(59)
mouse ... Acta2(11475)
rat ... Acta2(81633)

General description

其抗体(也称为抗α-Sm-1)对α-平滑肌肌动蛋白的单一异构体具有特异性。它在免疫印迹试验中可与α-平滑肌肌动蛋白发生特异性反应,并可在冷冻或福尔马林固定的、或石蜡包埋的组织切片中对平滑肌细胞进行标记。
单克隆抗肌动蛋白α-平滑肌(小鼠IgG2a同种型)源自小鼠骨髓瘤细胞和免疫BALB/c小鼠脾细胞融合产生的1A4杂交瘤

Immunogen

N-末端合成的α-平滑肌肌动蛋白十肽。

Application

Paraffin embedded sections of rat testis tissue grafts were immunohistochemically stained with mouse monoclonal anti-smooth muscle actin.
IHC analysis of x-gal stained muouse cardiac tissue was performed using the primary antibody, mouse monoclonal anti-smooth muscle actin to identify myofibroblasts.
使用单克隆抗ACTA2抗体对来自牛主动脉的平滑肌细胞进行免疫细胞化学分析。首先将细胞在玻璃盖玻片上生长,并在4%的50%丙酮/乙醇中固定10分钟。

Biochem/physiol Actions

在鸡和大鼠胚胎成肌过程中,平滑肌肌动蛋白可与肌节α-肌动蛋白进行瞬时共表达。在成年哺乳动物心脏的心室传导通道中,经证存在这种物质。它在平滑肌瘤、平滑肌肉瘤和平滑肌母细胞瘤以及部分横纹肌肉瘤中表达。

Physical form

溶解保存在0.01M磷酸缓冲盐,pH7.4, 含有1%牛血清白蛋白和15 mM叠氮化钠。

Other Notes

想要查看肌动蛋白抗体选择指南,请访问 www.sigmaaldrich.com/actin

Legal Information

Cy在 Amersham Biosciences Limited许可下销售。
Cy3 is a trademark of Cytiva

Disclaimer

除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。


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存储类别

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

低风险生物材料
常规特殊物品

此项目有



历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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商品

抗体可与特定的抗原结合,产生独特的抗体-抗原复合物。本文讨论这一连接键的性质,以及其作为分子标签的研究应用。

探索抗体处理基础知识,包括有关结构、类别和正常免疫球蛋白系列的技术信息。

Antibodies combine with specific antigens to generate an exclusive antibody-antigen complex. Learn about the nature of this bond and its use as a molecular tag for research.

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全球贸易项目编号

货号GTIN
C6198-.2ML04061833510964
C6198-100UL04061833510971