C8118
胃促胰酶 人
recombinant, expressed in Pichia pastoris
别名:
肥大细胞蛋白酶 I
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关于此项目
化学文摘社编号:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
Specific activity:
≥40 units/mg protein
Recombinant:
expressed in Pichia pastoris
Concentration:
125-400 μg/mL
recombinant
expressed in Pichia pastoris
Quality Level
form
liquid
specific activity
≥40 units/mg protein
mol wt
~37 kDa by SDS-PAGE
concentration
125-400 μg/mL
UniProt accession no.
application(s)
diagnostic assay manufacturing
shipped in
dry ice
storage temp.
−20°C
Gene Information
human ... CMA1(1215)
General description
糜蛋白酶是一种主要存在于肥大细胞的组织蛋白酶G样S1丝氨酸蛋白酶。它的分子量约为30 kDa，但在SDS-PAGE上的表观分子量约为37 kDa。
Application
在一项研究中，使用人糜酶来评估三七花提取物对心脏重构中TGFβ/ Smad信号转导途径的影响。 人糜酶也已被用于一项研究中，以研究链脲佐菌素处理的人糜酶转基因小鼠的血糖水平和存活率。
胸苷酶与血管紧张素II的产生和大内皮素的裂解有关。研究表明它可能与血管增生、心肌梗塞和皮炎有关。
Biochem/physiol Actions
该酶可迅速将血管紧张素 I 转化为血管紧张素 II。酶活性的最适 pH 值为 7.5 ~ 9.0。大豆胰蛋白酶抑制剂、苯甲基磺酰氟和糜蛋白酶抑制剂可抑制酶的活性。
Physical form
以溶于 20 mM Tris、0.8 MNaCl 和 25% 甘油的溶液 (pH 7.6) 形式提供
Other Notes
在 pH 7.8 和 25°C 条件下，1 个单元每分钟水解 1 μmol N-苯甲酰-L-酪氨酸乙酯 (BTEE)。用于测定酶活性的试验缓冲液含 27 mM Tris-HCl (pH 7.8)、150 mM NaCl 和 0.43 mM BTEE。
存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
常规特殊物品
此项目有
Ananya Roy et al.
The Journal of biological chemistry, 289(1), 237-250 (2013-11-22)
During infection and tissue damage, virulence factors and alarmins are pro-inflammatory and induce activation of various immune cells including macrophages and mast cells (MCs). Activated MCs instantly release preformed inflammatory mediators, including several proteases. The chymase mouse mast cell protease
Devandir Antonio de Souza et al.
PloS one, 7(7), e40790-e40790 (2012-07-21)
Tumor cells are surrounded by infiltrating inflammatory cells, such as lymphocytes, neutrophils, macrophages, and mast cells. A body of evidence indicates that mast cells are associated with various types of tumors. Although role of mast cells can be directly related
Jacco J de Haan et al.
American journal of physiology. Gastrointestinal and liver physiology, 305(5), G383-G391 (2013-07-03)
Nutritional stimulation of the cholecystokinin-1 receptor (CCK-1R) and nicotinic acetylcholine receptor (nAChR)-mediated vagal reflex was shown to reduce inflammation and preserve intestinal integrity. Mast cells are important early effectors of the innate immune response; therefore modulation of mucosal mast cells
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| C8118-50UG | 04061833521786 |