biological source
Porcine pancreas
Quality Level
grade
Proteomics Grade
form
lyophilized powder
specific activity
≥125 units/mg protein
mol wt
34,000 Da± 600
purified by
affinity chromatography
composition
Protein, 40-70%
foreign activity
carboxypeptidase A ≤1%, chymotrypsin ≤0.1%, trypsin ≤5%
storage temp.
−20°C
SMILES string
S(Cc3ccccc3)C(=O)C(NC(=O)C2N(CCC2)C(=O)C(NC(=O)C(NC(=O)CCC(=O)O)C)C)Cc1ccccc1
InChI
1S/C31H38N4O7S/c1-20(32-26(36)15-16-27(37)38)28(39)33-21(2)30(41)35-17-9-14-25(35)29(40)34-24(18-22-10-5-3-6-11-22)31(42)43-19-23-12-7-4-8-13-23/h3-8,10-13,20-21,24-25H,9,14-19H2,1-2H3,(H,32,36)(H,33,39)(H,34,40)(H,37,38)
InChI key
TWURVFFNODFJBJ-UHFFFAOYSA-N
Application
来自Sigma的酶已被用于开发均相时间分辨荧光（HTRF）检测法，以用于以小型化的高通量筛选形式测定羧肽酶B活性。它已被用于评估C-末端赖氨酸在人纤溶酶原结合至双歧杆菌过程中的影响。使用流式细胞术对用羧肽酶B（一种C-末端赖氨酸特异性内肽酶）处理的效果进行分析和检测。
羧肽酶B已被用于开发用于圈养和野生北极熊的非侵入性妊娠试验。羧肽酶B已被用于确定急性胰腺炎的新潜在生物标志物。
Biochem/physiol Actions
羧肽酶B是一种蛋白水解酶，能够快速水解肽键，从而从肽和蛋白质中释放某些羧基末端碱性氨基酸。其分子量为34,300±600 Da。其每摩尔含有一克不可透析的锌原子。金属螯合剂1,10-菲咯啉、8-羟基喹啉-5-磺酸、和2,2′-联吡啶可抑制该酶活性。
Packaging
基于蛋白含量进行包装
Physical form
含有HEPES缓冲盐、氯化锌和碳水化合物
Preparation Note
用蛋白酶抑制剂AEBSF进行处理，以消除丝氨酸蛋白酶活性。
Analysis Note
蛋白质由缩二脲进行确定。
Other Notes
在pH7.65，25℃条件下，一个单位每分钟可水解1.0 μmole马尿酰-L-精氨酸。
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
wgk
WGK 3
ppe
Eyeshields, Gloves
存储类别
11 - Combustible Solids
flash_point_f
No data available
flash_point_c
No data available
法规信息
低风险生物材料
动植物源性产品
此项目有
Laboratory diagnosis of acute pancreatitis: in search of the Holy Grail.
Lippi, G. et al.
Clinical Laboratory Science : Journal of the American Society For Medical Technology, 49, 18-31 (2012)
Marco Candela et al.
Journal of bacteriology, 189(16), 5929-5936 (2007-06-15)
Bifidobacteria constitute up to 3% of the total microbiota and represent one of the most important health-promoting bacterial groups of the human intestinal microflora. The presence of Bifidobacterium in the human gastrointestinal tract has been directly related to several health-promoting
Marc Ferrer et al.
Analytical biochemistry, 317(1), 94-98 (2003-05-06)
An epitope-unmasking, homogeneous time-resolved fluorescence (HTRF) assay has been developed for measuring carboxypeptidase B (CPB) activity in a miniaturized high-throughput screening format. The enzyme substrate (biotin-RYRGLMVGGVVR-OH) is cleaved by CPB at the C terminus, causing release of the C-terminal Arg
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| C9584-1MG | 04061833530894 |
| C9584-5MG | 04061833530900 |