Quality Level
usage
sufficient for 1,000 multiwell tests (in 96-well multiwell plates), sufficient for 100 standard tests (1 ml)
shipped in
dry ice
storage temp.
−20°C
Gene Information
human ... CASP3(836)
General description
Caspase 3 检测试剂盒（比色法）可在细胞溶解产物和半胱天冬酶3纯化制备物样本中快速、有效地检测半胱天冬酶3的活性。
Caspase 3 检测试剂盒（比色法）的检测原理为Caspase 3可水解乙酰基- Asp-Glu-Val-Asp对硝基苯胺（Ac-DEVD-pNA），释放对硝基苯胺（pNA）部分。然后在405 nm处测定对硝基苯胺(εmM=10.5)。再根据405 nm处的吸光度值，就可以计算出底物释放的pDNA浓度。此外，也可以将获得的校准曲线与pNA标准品曲线（试剂盒中包含pNA标准品）进行比较，计算出底物释放的pDNA浓度。
Caspase 3 检测试剂盒（比色法）的检测原理为Caspase 3可水解乙酰基- Asp-Glu-Val-Asp对硝基苯胺（Ac-DEVD-pNA），释放对硝基苯胺（pNA）部分。然后在405 nm处测定对硝基苯胺(εmM=10.5)。再根据405 nm处的吸光度值，就可以计算出底物释放的pDNA浓度。此外，也可以将获得的校准曲线与pNA标准品曲线（试剂盒中包含pNA标准品）进行比较，计算出底物释放的pDNA浓度。
Application
该检测既可采用(a)1 mL的检测体系由分光度计来测定；也可采用(b)100 µL检测体系在96孔板中由ELISA酶标仪来测量。
Other Notes
仅限使用17 megohm 水
在pH 7.4，25 °C条件下，1单位的Caspase 3每分钟裂解1.0 μmol的Ac-DEVD-pNA。
仅试剂盒组分
产品编号
说明
- Water (17-18 megaOhm) 125 mL
signalword
Danger
Hazard Classifications
Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 3 - STOT RE 2
存储类别
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
flash_point_f
415.4 °F - closed cup
flash_point_c
213 °C - closed cup
法规信息
常规特殊物品
危险化学品
此项目有
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全球贸易项目编号
| 货号 | GTIN |
|---|---|
| CASP3C-1KT | 04061835521760 |