MDA-MB-231 Cell Line human

MDA-MB-231 has been used to study:

• the changes in the morphological pattern and gene expression of cells when grown in the presence of sera positive for anti-carbonic anhydrase I (CA I) autoantibodies
• the effects of normothermic conditioned microwave irradiation on cultured cells using an irradiation system
• the transendothelial migration rates of the cells under different shear stress levels

STR-PCR Data: Amelogenin: X
CSF1PO: 12,13
D13S317: 13
D16S539: 12
D5S818: 12
D7S820: 8,9
THO1: 7,9.3
TPOX: 8,9
vWA: 15,18

Human Caucasian breast adenocarcinoma

Isolated from pleural effusions of a breast cancer patient.

L15+ 2mM Glutamine + 15% Foetal Bovine SerumFBS / FCS.

Split sub-confluent cultures (70-80%) 1:3 to 1:6, i.e. seeding at 3-6x10,000 cells/cm² using 0.05% Trypsin or Trypsin/EDTA; 37°C. CO₂ is not required.

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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). Use of this product by commercial organizations may require a license agreement. Commercial customers will be contacted by cellscreening@merckgroup.com to provide further information after the order request is submitted. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.